The Effect of Vitamin C Giving on the Degree of Leucocyte Infiltration and Edema Alveolar in Increasing the Level of Arterial Oxygen in White Rats (Rattus Norvegicus) Having Pulmonary Contusion

  • Nisma Yulius Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Muhammad Nuralim Mallapasi Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Rosie Rosie Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Jayarasti Kusumanegara Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Berti Nelwan Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Prihantono Prihantono Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Keywords: lung contusion, leucocyte infiltration, alveolar oedema, PaO2.


Lung contusion is the most common cases diagnosed at intrathoracic injury resulting from blunt trauma. Lung contusion usually caused excessive reactive oxygen species production, a condition that will trigger lipid peroxidase that will cause damage to the cell membrane and cell lysis. Vitamin C as an antioxidant will act as a ROS scavenger to prevent lipid peroxidase. The aim of this research is to examine the effectiveness of  vitamin C to increase the level of arterial blood oxygen and explain its pathomechanism. The research used experimental method conducted in the white rats (Rattus norvegicus). Study groups were divided into three groups, i.e.control group (CG, n=9), Lung contusion (LC, n=9), and pulmonary contusion given vitamin C (VC, n=9). For the experimental group, thoracic blunt trauma model was made by dropping the cylindrical iron load with the weight of (0.25 kg) on stainless steel pipe of the right hemithorax from the height of 80 cm (E = 1.96 J). For the VC group, the vitamin C of 200 mg/kg was injected 15 minutes after the intraperitoneal trauma. For 24 hours after the treatment, the examination of the blood gas analysis and pulmonary biopsy was conducted to find out oxygenation status and histopathological examination.The result of the analysis indicates that the pulmonary contusion given vitamin C has better arterial PaO2 value than the pulmonary group without being given vitamin C (p = 0,019). This is caused by the decrease of leucocyte infiltration degree (p = 0.011) and alveolar oedema (p = 0,050).  Vitamin C has  influence as an antioxidant towards changes in leukocyte infiltration rate, alveolar edema, and blood artery oxygen level at Sprague Dawley white rat (Rattus norvegicus) suffered from lung contusion.


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