Formulation and In-vitro Characterization of Metformin Hydrochloride-loaded Liposomes

Authors

  • Shrouk magdy Pharmaceutics Department, Faculty of Pharmacy, Deraya University, Minia, Egypt
  • Zeinab Fathalla Pharmaceutics Department, Faculty of Pharmacy, Minia University, Minia, Egypt
  • Eman Alaaeldin Pharmaceutics Department, Faculty of Pharmacy, Deraya University, Minia, Egypt
  • Heba F. Mansour Pharmaceutics Department, Faculty of Pharmacy, Minia University, Minia, Egypt

Keywords:

Metformin HCL, Liposome, ex-vivo permeation, skin, Drug formulations, stability study, parameter

Abstract

The aim of study. is to formulate Metformin hydrochloride-loaded novel liposomal vesicles and investigate their physical stability. A new metformin hydrochloride (Met-HCL) liposomal formulation was prepared  for topical delivery. Traditionally, the biguanide metformin could be categorized the first line in treatment of diabetes. The prepared metformin hydrochloride-loaded liposomal vesicles were investigated  for different in vitro characterisations. Eleven different formulations were developed adopting a thin film hydration method using different molar concentrations of Phospholipon® 90G, cholesterol and metformin hydrochloride. The effect of varying concentrations of Phospholipon® 90G, cholesterol and metformin hydrochloride on entrapment efficiency percent, ex-vivo skin permeation percentage, vesicle size and zeta potential was studied. Metformin-loaded liposome stability over a period of time 90 days was investigated. Results. The optimized metformin hydrochloride liposomes, F2, F6 and F11 were selecte. The selected formulations displayed highly efficient permeation percent via the excised mice skin 53±0.09 %, 30±0.4 % and 40±0.02 % respectively. The formulations showed EE % of  80±0.09 %, 28.6±0.02 % and 71.8±0.4 % respectively. Morphology of F2  liposomal surface revealed spherical three-dimensional structure. The stability study revealed about 10-23 percent drug leaching out of the vesicular liposomes (F2) within 90 days. Conclusion. Metformin hydrochloride-loaded liposomal vesicles can provide a potentially promising and convenient approach for topical delivery.

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Published

2021-02-25

How to Cite

magdy, S. ., Fathalla, Z. ., Alaaeldin, E. ., & F. Mansour, H. . (2021). Formulation and In-vitro Characterization of Metformin Hydrochloride-loaded Liposomes. International Journal of Sciences: Basic and Applied Research (IJSBAR), 56(1), 150–164. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/12330

Issue

Vol. 56 No. 1 (2021)

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