Formulation and In-vitro Characterization of Metformin Hydrochloride-loaded Liposomes
Keywords:Metformin HCL, Liposome, ex-vivo permeation, skin, Drug formulations, stability study, parameter
The aim of study. is to formulate Metformin hydrochloride-loaded novel liposomal vesicles and investigate their physical stability. A new metformin hydrochloride (Met-HCL) liposomal formulation was prepared for topical delivery. Traditionally, the biguanide metformin could be categorized the first line in treatment of diabetes. The prepared metformin hydrochloride-loaded liposomal vesicles were investigated for different in vitro characterisations. Eleven different formulations were developed adopting a thin film hydration method using different molar concentrations of Phospholipon® 90G, cholesterol and metformin hydrochloride. The effect of varying concentrations of Phospholipon® 90G, cholesterol and metformin hydrochloride on entrapment efficiency percent, ex-vivo skin permeation percentage, vesicle size and zeta potential was studied. Metformin-loaded liposome stability over a period of time 90 days was investigated. Results. The optimized metformin hydrochloride liposomes, F2, F6 and F11 were selecte. The selected formulations displayed highly efficient permeation percent via the excised mice skin 53±0.09 %, 30±0.4 % and 40±0.02 % respectively. The formulations showed EE % of 80±0.09 %, 28.6±0.02 % and 71.8±0.4 % respectively. Morphology of F2 liposomal surface revealed spherical three-dimensional structure. The stability study revealed about 10-23 percent drug leaching out of the vesicular liposomes (F2) within 90 days. Conclusion. Metformin hydrochloride-loaded liposomal vesicles can provide a potentially promising and convenient approach for topical delivery.
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