The Aqueous and Methanol Extracts of Ficus asperifolia (Moraceae) Improve Reproductive Hormones Profile in High Fat Diet Induced Obese Rats

Authors

  • Esther Ngadjui Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon
  • Georges Romeo Bonsou Fozin Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon
  • Aime Cesaire Momo Tetsatsi Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon
  • Modeste Wankeu-Nya Laboratory of Animal Biology and Physiology, Department of Animal Organisms Biology, University of Douala, Box 24157, Douala-Cameroun
  • Patrick Brice Defo Deeh Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon
  • Alango Pepin Nkeng-Efouet Department of Chemistry, Faculty of Science, University of Dschang, P.O. BOX 67 Dschang-Cameroon.
  • Telesphore Benoit Nguelefack Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon
  • Pierre Watcho Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon

Keywords:

Ficus asperifolia, estrus cycle, estradiol, progesterone, acute toxicity, high fat diet Type your

Abstract

Ficus asperifolia (Moraceae), is a plant used as fertility booster. We investigated the effects of F. asperifolia extracts on sex hormones profile in high-fat diet (HFD)?induced estrus cycle disturbances in rats. Female Wistar rats were fed either with HFD (n=161) or standard diet (n=8) for 10 weeks. After this period, 70 obese rats with abnormal estrus cycle were distributed into 14 groups of 5 animals each and treated for one and four weeks with distilled water (10 mg kg-1), Tween-80 (5%-10 mg kg-1), lutenyl (0.88 µg kg-1) and aqueous or methanol extract (100 or 500 mg kg-1) of F. asperifolia. At the end of each period, plasmatic estradiol and progesterone levels were determined. Acute toxicity parameters were also evaluated. HFD impaired reproductive hormones profile and estrous cycle after 10 weeks of treatment.F. asperifolia improved reproductive hormone profile by significantly increasing the plasmatic estradiol and progesterone levels. For instance, the plasmatic estradiol and progesterone levels were significantly increased (p<0.05-0.01) in rats treated with the aqueous (100 mg kg-1, four-weeks; 500 mg kg-1, one-week) or methanol (100 and 500 mg kg-1, four-weeks) extract of F. asperifolia. No toxic effects of F. asperifolia extracts were recorded. Our data support the traditional use of F. asperifolia as fertility enhancer.

References

. E.Q. Khor, J.P. Lim, L. Tay, A.Yeo, S. Yew, Y.Y. Ding, W.S. Lim. "Obesity definitions in sarcopenic obesity: differences in prevalence, agreement and association with muscle function". The Journal of Frailty & Aging, vol. 9, pp. 37-43, 2020.

. T.Fujiwara, R. Nakata, M. Ono, M. Mieda, H. Ando, T. Daikoku. H. Fujiwara. "Time restriction of food ıntake during the circadian cycle ıs a possible regulator of reproductive function in postadolescent female rats". Current Developments in Nutrition, Vol. 3, n°. 4, pp. nzy093, 2018.

. B. Luke. "Adverse effects of female obesity and interaction with race on reproductive potential". Fertility Sterility, vol. 107, n°. 4, pp.868-877, 2017.

. J.E. Schneider, J.D. Wise, N.A. Benton, J.M. Brozek, E. Keen-Rhinehart. "When do we eat? Ingestive behaviour, survival and reproductive success". Hormones and Behavior, vol. 64, pp. 702-728, 2013.

. M.H. Natalie, J.C. Kirstin, C.S. Delaney, E.S.W. Malgorzata. "High-fat diet exposure, regardless of induction of obesity, is associated with altered expression of genes critical to normal ovulatory function". Molecular and Cellular Endocrinology, vol. 10, pp. 1-9, 2017.

. J.L. Faulkner, S. Kennard, A.C. Huby, G. Antonova, Q. Lu, Jaffe, I.Z, Patel, et al. "Progesterone predisposes females to obesity-associated leptin-mediated endothelial dysfunction via upregulating endothelial MR (Mineralocorticoid Receptor) expression". Hypertension, vol. 74, pp. 678-686, 2019.

. H.H. Atteia, S. Alzahrani, N.A. El-Sherbeeny, A.M. Youssef, N.E. Farag, E.T. Mehanna, et al. "Evening primrose oil ameliorates hyperleptinemia and reproductive hormone disturbances in obese female rats: Impact on estrus cyclicity". Frontiers in Endocrinology (Lausanne), vol. 10, pp. 942, 2020.

. M.V. Bazzano, G.B. Sarrible, N. Martinez, M. Berón de Astrada, E.M. Elia. "Obesity alters the uterine environment before pregnancy". Journal of Nutrition and Biochemistry, vol. 62, pp. 181-191, 2018.

. C.Y. Wang, H.Y. Liu. "Epigenetic mechanism of obesity-induced male infertility". Zhonghua Nan Ke Xue, vol. 25, pp.1036-1039, 2019.

. M. Climént-Palmer, D. Spiegelhalter. "Hormone replacement therapy and the risk of breast cancer: How much should women worry about it"? Post Reproductive Health, vol. 25, pp. 175-178, 2019.

. K.M. Konda, M. Kabakura, B. Mbembe, Y. Itufa, K. Mahuku, M. Mafuta. "Plantes médicinales de traditions. Province de l'Equateur-R.D. Congo, in Kinshasa". Institut de Recherche en Sciences de la Santé de Kinshasa (I.R.S.S). [Medicinal plants of traditions. Province of Ecuador-R.D. Congo, in Kinshasa. Kinshasa Institute for Research in Health Sciences (I.R.S.S)]; pp. 419, 2012.

. P. Watcho, E. Ngadjui, P.A. Nkeng-efouet, T.B. Nguelefack, A. Kamanyi. "Reproductive effects of Ficus asperifolia (Moraceae) in female rats". African Health Sciences, vol. 9, pp. 49-53, 2009.

. P. Watcho, E. Ngadjui, P.A. Nkeng-efouet, T.B. Nguelefack, A. Kamanyi. "Evaluation of in vitro uterotonic activities of fruit extracts of Ficus asperifolia in rats". Evidence Based Complementary and Alternative Medecine, vol. 21, pp. 1-7, 2010.

. E. Ngadjui, P. Watcho, T.B. Nguelefack, A. Kamanyi. "Effects of Ficus asperifolia on normal rat estrus cyclicity". Asian Pacific Journal of Tropical Biomedicine, vol. 3, pp. 53-57, 2013.

. E. Ngadjui, P.A. Nkeng-Efouet, T.B. Nguelefack, A. Kamanyi, P. Watcho. "High Fat Diet-induced estrus cycle disruption: effects of Ficus asperifolia". Journal of Complementary and Integrative Medicine, vol. 12, pp. 205-15, 2015.

. S. De, Y.N. Dey, A.K. Ghosh. "Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphaphallus paeoniifolius (araceae) ". Indian Journal of Pharmaceutical and Biological Research, vol. 1, pp.150-157, 2010.

. EEC. Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Community, vol. 358, pp. 1-29, 1986.

. D.P.B. Deeh, M. Wankeu-Nya, E. Ngadjui, F.G.R. Bonsou, F.X. Kemka, A. Kamanyi, P. Watcho. "Palm oil diet-induced obesity impairs male rat reproductive performance". Annals of Reproductive Medicine and Treatment, vol. 2, pp. 1012, 2017a.

. D.P.B. Deeh, M. Wankeu-Nya, E. Ngadjui, F.G.R. Bonsou, F.X. Kemka, A. Kamanyi, et al. "The methanolic extract of Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats". Asian Pacific Journal of Reproduction, vol. 6, pp. 202-211, 2017b.

. Y. Lee, M.Y. Wang, T. Kakuma, Z.W. Wang, E. Babcock, K. McCorkle, et al. "Liporegulation in diet induced obesity. The antisteatotic role of hyperleptinemia". Journal of Biology Chemistry, vol. 276, pp. 5629-5635, 2001.

. G. Mausumi, D. Nirada, M. Rita, K.B. Mridul. "Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice". Contraception, vol. 76, pp. 482-485, 2007.

. C., Celik, M.R. Asoglu, L.S. Karakis, N. Findikli, M. Gultomruk, S. Cavkaytar, et al. "The impact of serum oestradiol concentration prior to progesterone administration on live birth rate in single vitrified-warmed blastocyst transfer cycles. Reproductive Biomedicine Online, vol. 39, pp. 1026-1033, 2019.

. M.A. Rezvanfar, A.R. Shahverdi, A. Ahmadi, M. Baeeri, A. Mohammadirad, M. Abdollahi. "Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicology and Applied Pharmacology, vol. 266, pp. 356-365, 2013.

. M. Sohrabi, A.M. Roushandeh, Z. Alizadeh, A. Vahidinia, M. Vahabian, M. Hosseini. "Effect of a high fat diet on ovary morphology, in vitro development, in vitro fertilisation rate and oocyte quality in mice". Singapore Medical Journal, vol. 56, pp. 573-579, 2015.

. J.H. Quennell, A.C. Mulligan, A. Tups. "Leptin indirectly regulates gonadotropin-releasing hormone neuronal function". Endocrinology, vol. 150, pp. 2805-2812, 2009.

. A. Hodonu, M. Escobar, L. Beach, J. Hunt, J. Rose. "Glycogen metabolism in mink uterine epithelial cells and its regulation by estradiol, progesterone and insulin". Theriogenology, vol. 130, pp. 62-70, 2019.

. J.T. Heiker, A. Kunath, J. Kosacka, G. Flehmig, A. Knigge, M. Kern et al. "Identification of genetic loci associated with different responses to high-fat diet-induced obesity in C57BL/6N and C57BL/6J substrains". Physiology and Genomics, vol. 46, pp. 377-384, 2014.

. P. Balasubramanian, L. Jagannathan, R.E. Mahaley, M. Subramanian, E.T. Gilbreath, et al. "High fat diet affects reproductive functions in female diet-induced obese and dietary resistant rats. Journal of Neuroendocrinology, vol. 24, pp. 748-755, 2012.

. N. Deblon, C. Veyrat-Durebex, L. Bourgoin, A. Caillon, A.L. Bussier, S. Petrosino, et al. "Mechanisms of the anti-obesity effects of oxytocin in diet-induced obese rats". PLoS One, vol. 6, pp. 255-265, 2011.

. Q.C. Ogbuji. "Obesity and reproductive performance in women". African Journal of Reproductive Health, vol. 14, pp. 143-151, 2010.

. T. Pantasri, R.J. Norman. The effects of being overweight and obese on female reproduction: a review. Gynecology Endocrinology, vol. 30, pp. 90-94, 2014.

. Y. Zhenxiang, X. Huali, Y. Xiaofeng, S. Dayun, L. Guangzhu. "Hypolipidemic effects of total flavonoide extracted from the leaves of Actinidia kolomikta in rats fed a high-fat diet". Iranian journal of basic medical sciences, 20: 1141-1148. 2017.

. C.E. Melton, P.S. Tucker, K.H. Fisher-Wellman, B.K. Schilling and R.J. Bloomer. "Acute exercise does not attenuate postprandial oxidative stress in prediabetic women". Physician and Sportsmedicine, 47: 519-21. 2009.

. J. Sarah. "Infertilité et ses traitements [Infertility and its treatments] ". Université de Strasbourg. 3: 71. 2014.

. National Pharmacopoeia Commission (NPC). "Pharmacopoeia of the People's Republic of China", China Medical Science and Technology Press. Beijing., 354-355. 2015.

. M. Singh, B.B. Saxena, R.S. Raghubanshi, W.J. Ledger, S.M. Harman and R.J. Leonard. "Biodegradable norethindrone (NET: cholesterol) contraceptive implants: phase II-A: a clinical study in women". Contraception., 55: 23-33. 1997.

. M.E. Skaznik-Wikiel, D.C. Swindle, A.A. Allshouse, A.J. Polotsky and J.L. Mc-Manaman. "High-fat diet causes subfertility and compromised ovarian function independent of obesity in mice". Biology of Reproduction, 94:108. 2016.

. G.N. Gupta, R. Landesman, B.B. Saxena, M. Singh, R.H. Asch, H.B. Croxatto, et al. "Multicenter clinical trial of implanted norethindrone pellets for long-acting contraception in women. Program for Applied Research on Fertility Regulation". Contraception., 30: 39-52. 1984.

. N.M. Orsi, N. Gopichandran, H.J. Leese, H.M. Picton and S.E. Harris. "Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle". Reproduction., 129: 219-228. 2005.

. S.J. Chandra, S.P. Ekambaram and V. Subramanian. "Acute and sub-acute toxicity studies on the polyherbal antidiabetic formulation Diakyur in experimental model". Journal of Health Sciences, 53: 245-249. 2007.

Downloads

Published

2021-05-11

How to Cite

Ngadjui, E. ., Fozin, G. R. B. ., Tetsatsi, A. C. M. ., Wankeu-Nya, M. ., Deeh, P. B. D. ., Nkeng-Efouet, A. P. ., Nguelefack, T. B. ., & Watcho, P. . (2021). The Aqueous and Methanol Extracts of Ficus asperifolia (Moraceae) Improve Reproductive Hormones Profile in High Fat Diet Induced Obese Rats. International Journal of Sciences: Basic and Applied Research (IJSBAR), 58(1), 118–130. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/12532

Issue

Vol. 58 No. 1 (2021)

Section

Articles

License

Authors who submit papers with this journal agree to the following terms.