Biochemical and Molecular Characterization of Gram-Negative Strain 37A Isolate from Spring Water Fountains
Keywords:
spring water, contamination, Erwinia persicina, pathogenAbstract
In this study, water samples from thirteen spring water fountains were collected in Burdur, Turkey. A gram-negative bacterial strain was isolated from cultures of water samples on Bunt and Rovira (BR) and 0.1 X Nutrient agar media. The isolated strain was characterized for cell wall structure, colony morphology, catalase expression, antibiotic sensitivity and 16S ribosomal RNA (rRNA) gene sequence. Results showed that the strain is gram-negative, catalase positive and rod shaped with pink colony morphology. The disc diffusion test for the strain revealed its sensitivity to cefoxitin, ofloxacin, tetracyclin, telithromycin, tobramycin, and sulfamethoxazole-trimethoprim antibiotics. Blast search for 16S rRNA gene sequence indicated that the strain is Erwinia persicina which is pathogenic strain for plants.
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