The Concentration of Hydrogen Peroxide, Percentage of Motility, Viability, Plasma Membrane Integrity, and Abnormality of Frozen Semen of Horned and Polled Bali Bulls
Keywords:
Horned and Polled Bali Bulls, Semen QualityAbstract
The aim of the study was to determine the frozen semen quality of horned and hornless (polled) Bali bull including hydrogen peroxide (H2O2) concentration, motility, viability, plasma membrane integrity, and abnormalities. This study used frozen semen from 2 horned and 2 polled Bali bulls aged 5 to 7 years. The concentration of H2O2 was measured using 2-7-Dichlorodihydrofluorescein Diacetate (DCHF-DA) dye, motility was assessed subjectively under a microscope, viability was assessed by eosin-negrosin staining, plasma membrane integrity was calculated using the Hypoosmotic Swelling Test (HOS-Test), and abnormalities were evaluated with Sperm Stein Ready to USE stain.
The results showed that there was no significant difference (P>0.05) between horned and polled based on the H2O2 concentration (2.37 ± 0.64 vs 2.65 ± 0.94), motility was (47.25 ± 5.04 vs 44.25 ± 8.12), plasma membrane integrity (50.62 ± 6.27 vs 52.75 ± 5.76), and abnormalities (15.90 ± 5.70 vs. 19.18 ± 6.39) but there was a significant difference (P<0.05) in the viability value (53.71 ± 8.56 vs. 64.09 ± 9.48). It can be concluded that the quality of frozen semen of horned and hornless (polled) Bali cattle based on H2O2 concentration, motility, plasma membrane integrity and abnormalities had the same conditions. The viability of spermatozoa from frozen semen of polled was better than that of horned Bali cattle.
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