Fabrication of a Nanoparticle-based Sensor for the Detection of Dengue Virus-3 in Aedes aegypti

Authors

  • Janell Contreras National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Ba
  • Lilia Fernando National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Ba
  • Evangelyn Alocilja Department of Biosystems and Agricultural Engineering, Michigan State University, 213 Farrall Hall, East Lansing, MI 48824-1323, USA
  • Ferdinand Salazar Medical Entomology Department, DOH
  • Brian Bacay Medical Entomology Department, DOH

Keywords:

colorimetric, dengue, DNAzymes, gold nanoparticles, nanobiosensor.

Abstract

The nanoparticle-based sensor was found to be serotype-specific for DENV-3 by demonstrating a positive result only with DENV-3. This was performed using extracted RNA of different dengue serotypes from A. aegypti. In this study, the fabricated nanoparticle-based sensor can detect target concentration for as low as 0.1 ?M using synthetic DENV-3 target and 5 x 102 PFU/mL using extracted RNA from A. aegypti.

The nanobiosensor presented in this study provides a simple, faster,

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Published

2016-04-29

How to Cite

Contreras, J., Fernando, L., Alocilja, E., Salazar, F., & Bacay, B. (2016). Fabrication of a Nanoparticle-based Sensor for the Detection of Dengue Virus-3 in Aedes aegypti. International Journal of Sciences: Basic and Applied Research (IJSBAR), 26(3), 138–157. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/5414

Issue

Vol. 26 No. 3 (2016)

Section

Articles

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