Dynamic Behavior of Primary Cilia During Cellular Migration

Authors

  • Kazuyuki Matsushima Saravio Central Institute, Saravio Cosmetics Ltd., 1356-6 Oaza Tsurumi, Beppu, Oita 874-0840, Japan
  • Kuniyoshi Kaseda Saravio Central Institute, Saravio Cosmetics Ltd., 1356-6 Oaza Tsurumi, Beppu, Oita 874-0840, Japan

Keywords:

Dermal fibroblast, dermal papilla cell, synoviocyte, migration, primary cilium, spheroid, wound healing.

Abstract

Dermal papilla cells (DPCs) play pivotal roles in hair follicle development and repetitive hair growth cycles, in which cellular migration is deeply involved. We previously suggested that primary cilia of DPCs regulate the hair follicle physiologies via intercellular signal transduction. However, the role of the primary cilia in cell migration has yet to be addressed. Here, immunofluorescence microscopic observations revealed that primary cilia of DPCs predominantly protruded toward the backward direction in the initial phase of cellular migration, while the organelle of non-migrating cells oriented randomly. In addition, the average length of the primary cilia became shorter upon the onset of the active motion, compared to those in multicellular spheroids. Moreover, the appearance frequency was dramatically decreased in migrating cells. Similar results were obtained using human dermal fibroblasts and articular synoviocytes. Collectively, our current findings suggest that those fibroblastic cells set the primary cilia to the backward direction in the initial phase of migration, along with the tuning of the length and appearance frequency of the sensory organelle. These regulations should be essential for proper cellular migration in tissue developments and wound-healing processes.

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Published

2017-07-14

How to Cite

Matsushima, K., & Kaseda, K. (2017). Dynamic Behavior of Primary Cilia During Cellular Migration. International Journal of Sciences: Basic and Applied Research (IJSBAR), 34(3), 1–12. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/7618

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