Close Genetic Relatedness of Chromis Fish (Abudefduf sp.) Between Indonesia and Korea Population
AbstractThe purpose of our study was to look closely relationship of Chromis fish taken from Korean waters and Indonesia based on Deoxyribose Nukleid Acid (DNA) mitochondrial marker. Samples of data taken from each of the 21 identified samples of the Abudefduf vaigiensis species, Abudefduf sexfasciatus and Abudefduf bengalensis. The samples taken were then extracted, amplified, and sequenced based on the mitochondrial DNA marker from the CYTB locus. The DNA sequence result is processed in MEGA 6.06 software to analyze the phylogenetic tree for determine close relatedness. The result showed there were 4 main clades including one clade outgrup explaining the chromis fish close relatedness i.e. 1) Abudefduf vaigiensis group, Abudefduf sexfasciatus and Abudefduf bengalensis, 2) Abudefduf Bengalensis group and 3) Abudefduf vaigiensis group from Buton waters location. The closest relatedness between Chromis fish found in Korea and Indonesia in the A. vaigiensis Korea and A. vaigiensis species in Sorong waters, ie, by the genetic distance of 0.0008. This genetic distance value in pairs of 10000 pairs of nucleotide have 8 pairs of different nucleotide.
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