Van A &B in Vancomycin-resistant Staphylococcus Aureus Isolated in Samarra City
Keywords:
Staphylococcus aureus.Abstract
A total of 110 isolates belonging to Staphylococcus aureus were obtained from different clinical samples of patients who were sleeping and visiting the Samarra General Hospital. From 25/4/2017 to 1/5/2017, a number of phenotypic and biochemical tests were adopted to distinguish bacteria from other bacterial species , The results showed that 25 isolates (22.7%) were resistant to vancomycin, . The putative presence of vanA gene was examined by PCR, using specific primers. Positive PCR amplifications were obtained in 3 isolates(12%) for vanA gene and the absence of the Van B gene. Vancomycin resistant staphylococcus aureus increased gradually in Samarra General Hospital and high dissemination of vanA gene, which encoded high resistance level to vancomycin. Continued surveillance is required to prevent further spread of these serious resistances. Van A & B genes were detected using PCR technique. This is evidence of high resistance to vancomycin in Samarra General Hospital.
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