Rice Resistance-Treated with Endophyte Fungi Against Drought Stress

Authors

  • Rangga Yuspradana Department of Soil Science and Land Resources, Faculty Of Agriculture, Bogor Agricultural University, Dramaga, Bogor 16680, Indonesia
  • Suryo Wiyono Department of Plant Protection, Faculty Of Agriculture, Bogor Agriciultural University, Dramaga, Bogor 16680, Indonesia
  • Rahayu Widyastuti Department of Soil Science and Land Resources, Faculty Of Agriculture, Bogor Agricultural University, Dramaga, Bogor 16680, Indonesia

Keywords:

rice plant, drought stress conditions, endophyte fungi, growth and biological parameter.

Abstract

Drought problem is a major inhibiting factor for the crops producing and growing, one of them rice plants that require a lot of water supply. Endophyte fungi a potential for increasing plant resistance against drought. This study aims to determine the endophyte fungi enhancing growth of rice plants in drought stress conditions with three treatments of field capacity. Type of endophyte fungi used in this study is Acremonium sp., Curvularia sp., Penicillium sp., and Nigrospora sp. We compare some growth and biological parameters such as germination capability, ability of colonization, stomatal density, plant height and number of tillers, plant weight, root length, root volume, ratio root : shoot and leaf color. Statistical analysis using a two-factor randomized block design. The result showed that endophyte fungi treatment increased stomatal density, plant height, number of tillers, dry weight, root length, root volume, dry root weight, and leaf color index when compared with endophyte treatment in low water capacity treatment. The treatment of endophyte fungi curvularia sp. gave the highest results on the parameters of stomatal density, dry weight of stem, root length, root dry weight significantly different from treatment without endophytes. in contrast, the parameters of the number of open stomata are not significantly different with no endophytes so that the fungi can increase help rice plant to growth in under drought stress conditions.

References

J. B. Passioura. “Drought and drought tolerance”. Plant Growth Regulation 20(2): 79-83, 1996.

E. Sulistyono, D. Sopandie, M.A Chozin, Suwarno. “Adaptation of upland rice to shade: morphological and physiological approaches”. J. Comm. Ag. 4 (2):62 – 67, 2007.

S. Sarkarung, G. Pantuwan, S. Pusphavesa, P. Tanupan. “Germplasm Development for Rainfed Lowland Ecosystems”. In Proc.Breeding Strategies for Rainfed Lowland Rice in Drought-prone Environments, 1996 , pp: 43–49

W. Prihatiningtyas, M. S. H. Wahyuningsih. “Prospects of Endophyte Microbes as a Source of Bioactive Compounds”. Internet: https://mot.farmasi.ugm.ac.id/files/29Review%20Endophyte.pdf, [date and month not available], 2011 [Mar. 17, 2015].

K. Saikkonen, S. H. Faeth, M. Helander, T. J. Sullivan. Fungal Endophytes: A Continum of Interactions with Host Plants. Annu Rev Ecol Syst 29 vol 3, pp. 19–43, 1988.

Z. Mirzahossini, S. Leila, M. R. Sabzalian, M. S. Tahrini. ABC transporter and metallothionein expression affected by Ni and epichloe endophyte infection in tall fescue. J Ecoxitology and Environ Safety 120:13-19, 2014.

S. Li, B. Ning, L. Yueying, M. Lianju, X. Shigang, L. Zhang. Growth, photosynthesis and antioxidant responses of endophyte infected and non-infected rice under lead stress conditions. J of Hazardous Materials vol 213, pp. 55– 61, 2012.

(Khan et al. 2014) A. L. Khan, J.H. Shin, H. Y. Jung., I. J. Lee. Regulations of capsaicin synthesis in Capsicum annum L. by Penicillium resedanum LK6 during drought conditions. J Sci Hor vol. 175, pp. 167-173, 2014.

ISTA. International Rules for Seed Testing:Ed. 2006. The international seed testing association. Bassersdorf, CH: ISTA, 1972.

E. P. Ramdan. Exploration of endophyte fungi as biological controlling agent Phytophthora Capsici L. on pepper plant” Thesis, Bogor Agricultural University, Bogor, Indonesia, 2014.

T. Setiawan, S. Djafar. Effect of drought stress on proline accumulation of patchouli plant (Pogostemon cablin Benth.) J. Ilmu Pertanian 15, pp. 85 – 99, 2012.

A. D. H Totok, A. H. Rahayu AH. Analysis of N uptake efficiency, growth, and yield of some new superior soybean cultivar with drought stress and biological fertilizer. J. Agrosains 6 vol. 20, pp:70-74 ,2004.

M. D. Richardson, C.W. Bacon, C. S. Hoveland. “The effect of endophyte removal on gas exchange in tall fescue” In proc. Proceed of the International Symposium on Acremonium/grass Interactions. 1990, pp.189-193.

R. S. Redman, Y.O. Kim, C. J. D. A. Woodward, C. Greer, L. Espino. “Increased fitness of rice plants to abiotik stress via habitat adapted symbiosis: A Strategy for Mitigating Impacts of Climate Change”. J. PLoS ONE 6 vol. 7 eISSN: 14823, 2011.

G. Colla, Y. Rouphael, P. Bonini, M. Cardarelli. “Coating seeds with endophyte fungi enhances growth, nutrient uptake, yield and grain quality of winter wheat”. Inter. J. of Pla. Production, 9 vol. 2 pp: 171-189, 2015.

A. L. Khan, S. A. Gilani, M. Waqas, K. Hosni, S. Khiziri, Y. Kim, L. Ali, S. Kang, S. Asaf, R. Shahzad, J. Hussain, J. Lee, A. Harrassi. “Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress”. J. of Zhejiang Univ. Sci. B (Biomed. & Biotechnol.) 18 vol. 2, pp: 125-137, 2017.

P. Goransson, P. A. Olsson, J. Postma, U. F. Grerup. “Colonisation by arbuscular mycorrhizal and fine endophyte fungi in four woodland grasses – variation in relation to pH and aluminium”. J. Soil Bio. & Biochem. 40 vol. 9 pp: 2260-2265, 2008.

J. Pitt, A. Hocking. Fungi and Food Spoilage. (3rd edition). New York (US):Splinger, 2009, pp.194.

W. A. D. K. Wijesooriya, N. Deshappriya. “An inoculum of endophyte fungi for improved growth of a traditional rice variety in Sri Lanka”. J. Trop. Pla. Res. 3 vol. 3, pp: 470–480, 2016..

L. H. Bhattacharya, G. Borah, V. Parkash, P. N. Bhattacharya. “Fungal endophytes associated with the ethnomedicinal plant Meyna spinosa Roxb”. J. Cur. Life Sci. 3 vol 1, pp:1-5, 2016.

R. C. Njokoucha, C. O. C. Agwu, C. E. A. Okezie. “Effects of weather conditions on selected airborne fungal spores in the southern part of the state of Enugu, Nigeria”. J. Grana 56 vol. (4), pp: 263-272, 2016.

L. Zakariah, A. S. Yaakop, B. Salleh, M. Zakariah. “Endophyte fungi from paddy”. J. Trop. Life Sci. Res. 21 vol. 1, pp: 101–107, 2010.

S. K. Gond, A. Mishra, V.K. Sharma, S. K. Verma, J. Kumar, R. N Kharwar, A. Kumar. “Diversity and antimicrobial activity of endophyte fungi isolated from Nyctanthes arbor-tristis, a well-known medicinal plant of India”. J. Micro Sci. 53, pp: 113–121, 2012..

J. A. Lake, F.I. Woodward. “Response of stomatal numbers to CO2 and humidity:without endophyte by transpiration rate and abscisic acid”. J. New Phyto. 179, pp: 397–404, 2008.

P. Priyadharsini P, T. Muthkumar. “The root endophyte fungi Curvularia geniculata from Parthenium hysterophorus roots improves plant growth through phosphate solubilization and phytohormon production”. J. Fungal Eco. 27, pp: 69-77, 2017.

S. Muthulaksmi, V. Pandiyarajan. “Effect of IAA on the Growth, Physiological and Biochemical Characteristics in Catharanthus roseus (L). Inter. J. Of Sci. Res. 4 vol. 3, pp: 442-448, 2013.

A. G. Babu, S. W. Kim, D. R. Yadav, U. Hyum, M. Andhikari, Y. S. Lee. “Penicillium menonorum: a novel fungi to promote growth & nutrient management in cucumber Plants”. J. Myco. 43 vol. 1, pp: 49–56, 2015.

J. M. Ruiz, R. Azcon. “Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status”. J. Physiol. Pla. 95 vol. 4, pp: 72-78, 1995.

W.H GeraHol, E. DelaPena, M. Moens, R. Cook. “Interaction between a fungal endophyte and root herbivores of Ammophila arenaria”. J. Basic and App. Ecol. Vol. 8, pp: 500-509, 2007.

B. R. Murphy, L. M. Nieto, F.M. Doohan, T.R. Hodkinson. “Fungal endophytes enhance agronomically important traits in severely drought-stressed barley”. J. of Agro. Crop. Sci. 201, pp: 419–427, 2015.

M. Rai, A. Varma. “Arbuscular mycorrhiza-like biotechnological potential of Piriformospora indica, which promotes the growth of Adhatoda Vasica Nees. Electron J. Biotechnol. 8 vol. 1, pp: 107-112, 2005..

Y. Feng, D. Shen, W. Song. “Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthesis”. J. of App. Microbiol. 100, pp: 938–945, 2005.

J.M. Ruiz, R. Azcon. “Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status”. J. Physiol. Pla. 95 vol. 4, pp: 72-78, 1995.

M. Waqas M, A. L. Khan, M. Kamran, M. Hamayun, S. Kang, Y. Kim, I. Lee. “Endophyte fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress”. J Molecules 17,pp: :10754-10773, 2012.

T.C. De-Souza, P. C. Magalhaes, E. M. Castro. “Corn root morphoanatomy at different development stages and yield under water stress”. J. Pesq. Agropec. Bras.” 51 vol. 4, pp::330-339, 2016.

J. M. Henson, K.B. Sheehan, R. J. Rodriguez, R. S. Redman. “Curvularia strains and their use to confer stress tolerance and/or growth enhancement in plants”. U.S. Patent 7 906 313 B2, Mar. 15, 2011.

B. Kurniasih, F. Wulandhany. A roll-up of leaves, canopy and root growth of several varieties of upland rice in different water stress conditions. J. Agrivita 31, pp: 118-128, 2009.

Downloads

Published

2017-10-09

How to Cite

Yuspradana, R., Wiyono, S., & Widyastuti, R. (2017). Rice Resistance-Treated with Endophyte Fungi Against Drought Stress. International Journal of Sciences: Basic and Applied Research (IJSBAR), 36(3), 256–270. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/8062

Issue

Section

Articles