Immunomodulatory Effects of Neem (Azadirachta indica) Leaf Aqueous Extracts in Cockerels Vaccinated and Experimentally Infected with Infectious Bursal Disease Virus

Authors

  • Garba S. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Mera U. M. Department of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Musa U. Department of Veterinary Pathology, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Onifade K. I. Department of Veterinary Pharmacology and Toxicology, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Raji A. A. Department of Veterinary Pathology, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Jibrin M. S. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Shehu Z. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Ahmad U. S. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Rambo U. G. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Mungadi H. U. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Yakubu Y. Department of Veterinary Public Health and Preventive Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Mshelia P. C. Veterinary Teaching Hospital, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Shu’aibu A. B. Department of Veterinary Physiology and Pharmacology, Usmanu Danfodiyo University, Sokoto, Nigeria

Keywords:

Infectious bursal disease, neem leaf aqueous extracts, immunomodulation, vaccine, challenge, antibody, cockerels.

Abstract

The aim of the present work was to study the immunomodulatory potentials of aqueous extract of Neem (Azadirachta indica) leaf in cockerels vaccinated and/ or infected with infectious bursal disease virus (IBDV). Four hundred and eighty (480) day old cockerels were used and allocated into 8 groups. The birds were grouped as vaccinated/ unvaccinated, challenged/ unchallenged, neem leaf treated/ untreated groups. The IBD vaccines (intermediate plus strain) were given at 14 and 28 days of age while the experimental infection using very virulent IBD virus (vvIBDV) was inoculated at 35 days of age and the extracts were given from day old to 6 week old.

Serum samples were collected on first day and on weekly intervals while post challenge, onset of the disease, clinical signs and mortality rate were recorded. The results obtained showed higher antibody titre, faster seroconversion, mild clinical sign and very low mortality in the neem leaf treated groups. These results indicated that the neem leaf aqueous extract has immunomodulatory potentials by increasing the antibody titre post vaccination and the ability to prevent mortality.

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Published

2018-02-03

How to Cite

S., G., U. M., M., U., M., K. I., O., A. A., R., M. S., J., Z., S., U. S., A., U. G., R., H. U., M., Y., Y., P. C., M., & A. B., S. (2018). Immunomodulatory Effects of Neem (Azadirachta indica) Leaf Aqueous Extracts in Cockerels Vaccinated and Experimentally Infected with Infectious Bursal Disease Virus. International Journal of Sciences: Basic and Applied Research (IJSBAR), 37(2), 72–89. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/8348

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Vol. 37 No. 2 (2018)

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