The Study of Trigona spp Propolis Against Pro-Inflammatory Cytokine Production in Spraque Dawley Rats Macrophages Infected with Staphylococcus aureus
Keywords:
propolis Trigona spp, pro-inflammatory cytokine, macrophages.Abstract
Propolis helps boost the immune system naturally because propolis is rich in bioactive compounds and can help increase the production and activity of immune cells. This product has attracted the interest of researchers in recent decades because of the properties of biological and pharmacological, among others such as antimicrobial, antitumor, anti-inflammatory, antioxidant and immunomodulatory. One of bee species that potentially produce propolis in Indonesia is a local bee species Trigona spp. This study aimed to to determine the immunomodulatory effects of ethanol extract of propolis on the Sprague Dawley rats were infected by Staphylococcus aureus.contained in propolis liquid. The effects of propolis were analyzed using the macrophage activity as determined by the phagocytic activity and by the production of nitric oxide (NO) in Sprague Dawley rat peritoneal macrophages and the production of antibodies.
Identification of the phytochemical compounds was undertaken to determine the bioactive compounds contained in propolis liquid. The effects of propolis were analyzed using the macrophage activity as determined by the phagocytic activity and by the production of nitric oxide (NO) in Sprague Dawley rat peritoneal macrophages and the production of antibodies. The results showed that the liquid propolis produces (a) an increase in the phagocytic index, (b) a significant increase in NO production, and (c) an increase over the production of IgG antibodies. This study indicates that the ethanolic extract of propolis of Trigona spp. is able to activate macrophages and promote the production of antibodies. The combination of these results suggests that this extract has an immunomodulatory effect and can boost the immune response.
References
. A Ratman and Fedik. 2003. Immunological Method. Surabaya (ID): Airlangga University Press. Indonesia.
. D Toro-Arreola, E Flores-Torales, C Torres-Lozano, AD Toro-Arreola, K Tostado-Pelayo, MG Ramirez-Duen˜as, A Daneri-Navarro. 2005. Effect of D-limonene on immune response in BALB/c mice with lymphoma. International Immunopharmacology. 5(5):829-838.
. M Draganova-Filipova, M Nikolova, A Mihova, L Peychev. 2010. A pilot study on the immunomodulatory effect of Bulgarian propolis. Biotechnology & Biotechnological Equipment. 24:sup1, 119-124. doi:10.1080/13102818.2010.10817824.
. GK Hansson. 2001. Immune mechanisms in atherosclerosis. Nature Immunology. 12:204-209.
. A Karumi, M Sawako, MY Mari. 2005. Kaempferol, a tea flavonol, effect of interleukin-2 signal transduction of human T cell leukemia. JARQ. 39(3):175-9.
. AM Knab, DC Nieman, ND Gillitt, RA Shanely, L Cialdella-Kam, DA Henson, W Sha. 2013. Effects of a flavonoid-rich juice on inflammation, oxidative stress, and immunity in elite swimmers: A Metabolomics-Based Approach. Sport Nutrition and Exercise Metabolism. 23:150-160.
. NA Andreollo, EF dos Santos, MR Araujo, LR Lopes. 2012. Rat’s age versus human’s age: What is The Relationship ?. Review Article. ABCD Arq Bras Cir Dig. 25(1):49-51.
. WT Frederer. 1967. Experimental design: theory and application. Calcutta(IN): Oxford & IBH Publ. Co.
. PG Reeves, H Forrest, Nielsen, C George, Fahey. 1993. AIN-93 purified diets for laboratory rodents: final report of the american institut of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet, committee report. Journal of Nutrition. 123:1939-1951.
. JH Park, JK Lee, HS Kim, ST Chung, JH Eom, KA Kim, SJ Chung, SY Paik, HY Oh. 2004. Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice. Int Immunopharmacol. 4(3):429-436.
. N Kalsum, A Sulaeman, B Setiawan, IWT Wibawan. 2016. Phytochemical Profiles of Propolis Trigona spp. from Three Regions in Indonesia Using GC-MS. Journal of Biology, Agriculture and Healthcare (JBAH). 6:14
. KG Baratawidjadja. 2014. Basic immunology. Edition 11. Jakarta (ID): FKUI
. E Middleton, C Kandaswami, CT Theoharides. 2000. The effect of plant flavonoids on mammalian cells: Implications for Inflammation, Heart Disease, and Cancer. Pharmacological Reviews. 52(4):673-751.
. AK Abbas, AH Lichtman, S Pillai. 2012. Cellular and molecular immunology. 7th ed. Philadelphia(US): Saunders Elsevier.
. CM Lappas and NT Lappas. 2012. D-limonene modulates T lymphocyte activity and viability. Cellular Immunology. 279:30-41.
. M Hamada, K Uezu, J Matsushita, S Yamamoto, Y Kishino. 2002. Distribution and immune responses resulting from oral administration of D-limonene in rats. J Nutr Sci Vitaminol. 48(2):155-60.
. WJ Yoon, NH Lee, CG Hyun. 2010. Limonene suppresses lipopolysaccharide-induced production of nitric oxide, prostaglandin E and pro-inflammatory cytokines in RAW 264.7 macrophages. J. Oleo Sci. 59:415-421.
. JM Sforcin. 2007. Propolis and the immune system: a review. Journal of Ethnopharmacology. 113:1–14.
. AL Teixeira, R Ribeiro, D Cardoso, D Pinto, F Lobo, A Fraga, F Pina, F Calais-da-Silva, R Medeiros. 2008. Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients. Clin. Cancer Res. 14:3367–3371.
. J.D. Fraser and T Proft. “The bacterial superantigen and superantigen-like proteins.” Immunol Rev. vol. 225, pp.226-243, Oct. 2008. doi: 10.1111/j.1600-065X.2008.00681.x
. T Krakauer, MJ Buckley MJ, D Fisher. 2010. Pro-inflammatory mediators of toxic shock and their correlation to lethality. Mediators of Inflammation. Oct. 2010 Article ID 517594. http://dx.doi.org/10.1155/2010/517594
. J.J. Cheng. "Caffeic acid phenethyl ester suppresses IFN-γ-induced stat1 activation and gene expression in endothelial cells.” J Chin Med. vol.23, pp.103-112, Mei 2012.
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