Expression of Toll-like Receptor-4 (tlr-4) in Balb/c Mice Induced by Salmonella Typhi

Authors

  • Fadhilah Fadhilah Doctoral programme, Faculty of Medicine, Hasanuddin University, Indonesia
  • Gemini Alam Faculty of Pharmacy, Hasanuddin University, Indonesia
  • Mochammad Hatta Faculty of Medicine, Hasanuddin University, Indonesia
  • Rosdiana Natzir Faculty of Medicine, Hasanuddin University, Indonesia
  • Burhanuddin Bahar Department of Public Health Nutrition, Hasanuddin University, Makassar, South Sulawesi, Indonesia

Keywords:

Toll-like receptor-4 (TLR-4), Salmonella typhi, Real Time- Polymerase Chain Reaction (RT-PCR).

Abstract

Salmonella typhi is a gram negative bacteria that causes systemic infections and typhoid fever in humans. It has caused many deaths in developing countries.  WHO (World Health Organization) estimated the incidence rate worldwide approximately 17 million per year with 600,000 deaths  and 70% of them occurred in Asia (WHO, 2008). By Toll-like receptor  4 (TLR-4), macrophages can recognize lipopolysaccharides (LPS) present in S. typhi. The bond of LPS and TLR-4 that activates MyD88 plays an important role in controlling bacterial exponential growth. This study aimed to analyze the TLR-4 mRNA in Balb/c mice induced by S.typhi. This study was a laboratory experimental research on Balb/c mice with simple randomized design. Mice blood was taken before and after  induction by 103 CFU of S.typhi. Then the blood samples were examined at Microbiology Laboratory in Faculty of Medicine, Hasanuddin University to measure expression of Toll-like receptor-4 (TLR-4) mRNA using Real Time Polymerase Chain Reaction (RT-PCR). The result of this study showed that there were enhancement of TLR-4 expression from 20 samples obtained. The data indicated that S.typhi induction can increase TLR-4 expression.

References

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Published

2017-11-13

How to Cite

Fadhilah, F., Alam, G., Hatta, M., Natzir, R., & Bahar, B. (2017). Expression of Toll-like Receptor-4 (tlr-4) in Balb/c Mice Induced by Salmonella Typhi. International Journal of Sciences: Basic and Applied Research (IJSBAR), 36(6), 42–46. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/8382

Issue

Vol. 36 No. 6 (2017)

Section

Articles

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