A Study on Germination and Early Seedling Growth of Zea mays L.in Hibiscus sabdariffa Linn. Extract Treated Medium

Authors

  • Mbosowo M. Etukudo Department of Biology, Federal University Otuoke, PMB 126, Bayelsa State, Nigeria
  • Anthony Egbe Department of Biological Sciences, Cross River University of Technology, Calabar, Cross River State, Nigeria

Keywords:

Germination, Growth, Zea mays, Hibiscus sabdariffa, Extract .

Abstract

Germination and early seedling growth of Zea mays   in  aqueous extract of Hibiscus sabdariffa calyces treated medium  was conducted in the laboratory. The concentration levels of 20, 40, 60, 80, and 100%  were prepared through serial dilutions, while 0% (distilled water) was used as control. Elemental composition of calyces of H. sabdariffa was determined using standard procedures. 10 seeds of the test crop were sown in sterilized Petri dishes containing two sterile What-Man’s filter paper per treatment. Percentage germination and coefficient of velocity of germination counts were recorded at 24, 48, 72, and 96 hours after sowing all the seeds.  Growth parameters such as shoot length, root length, fresh weight and dry weight of the seedlings were measured after 20 days. Elemental analysis of  aqueous extract of H. sabdariffa calyces indicated the presence calcium, magnesium, sodium, potassium, phosphorus, nitrogen, iron, manganese, copper, zinc and lead in varied concentrations. The values of coefficient of velocity of germination and percentage germination of Zea mays  were significantly (P < 0.05) decreased with increase in the concentration of H. sabdariffa  extract, except at 20% concentration level of H. sabdariffa  extract where slight stimulations were recorded in comparison with the control treatment. Similarly, the shoot length, root length, fresh weight and dry weight of maize seedlings were significantly (P < 0.05) decreased with increase in the concentration of H. sabdariffa  extract, except at 20% concentration level of H. sabdariffa  extract where the shoot length was slightly stimulated above that of the control treatment. This study suggests that  aqueous extract of Hibiscus sabdariffa calyces could negatively affect the germination and early seedling growth of Z. mays.

References

G. Oboh and C.A Elusiyan. “ Nutrient composition and antimicrobial activity of sorrel drinks (soborodo).” J Med. Food, vol. 7 no. 3, pp. 340-342, 2004.

N. C McClintock and M. El Tahir. “Hibiscus sabdariffa L.” In Grubben G.J.H and Denton O.A Ed. Plant Resources of Tropical Africa 2. Vegetables. PROTA Foundation, Wageningen Netherlands/Backhuys Publishers: Leiden, Netherlands/CTA, Wageningen, Netherlands, 2004, pp. 321-329.

O. B Dokosi (1998). Herbs of Ghana. Ghana Universities Press: Ghana, pp. 141 157.

R.R Schippers. African Indigenous Vegetables. An overview of the Cultivated Species. Natural Resources Institute, Chatham:, UK, 2000, pp. 119-133.

O.I Adejumo, A.A Alfa and A. Mohammed. “Physical Properties of Kano White Variety of Bambara Groundnut.” Nigerian Academic Forum, vol. 12, no.1, pp. 68-77, 2007.

Y.C Chang, K..X Huang ,A.C Huang, Y.C Ho and C.J Wang. “Hibiscus anthocyanins-rich extract inhibited LDL oxidation and oxLDL-mediated macrophages apoptosis.” Food and Chemical Toxicology, vol. 44, no.7, pp. 1015–1023, 2006.

K.M Elamin, H.E Hassan, H.O Abdalla, O.H Arabi, and A.A.T Eldar.”Effect of feeding crushed Roselle seed (Hibiscus sabdariffa L.) (Karkadeh) on carcass characteristics of Sudan desert sheep.” Asian Journal of Animal Science, vol. 6, no. 5, pp. 240–248, 2012.

A. Ismail, E.H.K. Ikram and H.S.M. Nazri. “Roselle (Hibiscus sabdariffa L.) seeds nutritional composition protein quality and health benefits.” Food, vol. 2, no. 1, pp. 1-16, 2008.

.M. Nzikou, G. Bouanga-Kalou, L. Matos, F.B. Ganongo-Po, P.S. Mboungou-Mboussi, F.E. Moutoula, et al. “Characteristics and nutritional evaluation of seed oil from Roselle (Hibiscus sabdariffa L.) in Congo-Brazzaville. Current Research Journal of Biological Sciences, vol. 3, no. 2, pp. 141-146, 2011.

C.C Chen, J.D Hsu, S.F Wang, H.C Chiang, M.Y Yang, E.S Kao, E.S., et al. “Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits.” Journal of Agricultural and Food Chemistry, vol. 51, no.18, 5472–5477, 2003.

S. Gosain, R. Ircchiaya, P.C Sharma, S. Thareja, A. Kalra, A. Deep et al., “Hypolipidemic effect of ethanolic extract from the leaves of Hibiscus sabdariffa L. in hyperlipidemic rats”. Acta Poloniae Pharmaceutica, vol. 67, no.2, pp. 179–184, 2010.

D.I McKay, C.Y, Chen, E. Saltzman, and J.B Blumberg. “Hibiscus sabdariffa L. tea (tisane) lowers blood pressure in prehypertensive and mildly hypertensive adults.” The Journal of Nutrition, vol. 140, pp. 298-303, 2010.

A.C Dutta.. Botany for Degree Students. Oxford University Press: India, 2012, pp. 125-137.

M.M Etukudo, E.M.I Roberts, and B.O Ilesanmi. “Some regulation of In vitro Growth of Bush mango- Irvingia wombolu Mildbr (Irvingiaceae).” World Research Journal of Agricultural Sciences, vol.1, no.2, pp. 7- 12 ,2014.

M.M Etukudo, A. Hamilton-Amachree and E.M.I Roberts. “Eco-physiological Studies of Elemental and Proximate Contents of Gnetum africanum Welw and Telfairia occidentalis Hook seeds from two Ecological Zones of Akwa Ibom State.” European International Journal of Science and Technology, vol. 4, no.6, pp.:47-53, 2015.

M.M Etukudo, S.E Osim and A. Egbe, “Assessment Of fresh and dry cassava peels for chemical nutrients potentials or growth of Telfairia occidentalis Hook In Tropical soil.” Journal of Advanced Scientific Research, vol. 7, no.1, pp. 49-52, 2016.

S.U Chon, S.K. Choi, S.D. Jung, B.S. Pyo and M. Kims. “Effects of alfalfa leaf extracts and phenolic allelochemicalson early seedling growth and root morphology of alfalfa and barnyard grass.” Crop Prot., vol.5, pp. 1077-1082, 2002.

S. Sadeghi, A. Rahnavard and Z.Y Azhrafi. “Allelopathic effect of Helianthus annus (sunflower) on Solanum nigrum (Black Nightshade) seed germination and growth in laboratory condition.” Journal of Horticultural Science and Ornamental Plants, vol. 2 no. 1, pp. 32–37, 2010.

P. Oudhia. “Allelopathic effect of some obnoxious weeds on germination of soybean.” Indian Journal of Plant Physiology, vol.5, no.3, 295–296, 2000a.

P. Oudhia. “Germination and seedling vigour of Kodomillet as affected by allelopathy of Ipomoea carnea Jacq.” Indian Journal of Plant Physiology, vol. 5 no. 4, pp. 383–384, 2000b

P.S Vankar and J. Srivastava. “Comparative Study of Total Phenol, Flavonoid Contents and Antioxidant Activity in Canna indica and Hibiscus rosa sinensis, Prospective Natural Food Dyes.” International Journal of Food Engneering, vol. 4, no. 3, pp.,1–17, 2008.

R.R Barkosky and F.A Einhellig. “Allelopathic interference of plant -water relationships parahydroxybenzoic acid.” Botanical Bulletin-Academia Sinica Taipei, vol. 44, pp. 53–58, 2003.

R. Naz, and A. Bano. “Effects of allelochemical extracts from medicinal plants on physiological and biochemical mechanisms of maize (Zea mays L.) seedlings.” International Journal of Agronomy and Agricultural Research, vol. 5, no. 2, pp.31-39, 2014

A.O.A.C., Association of Official Analytical Chemist. Methods of analysis. 16h Edition, Washington DC Press: U.S.A, 1999.

I.U Obi. Statistical methods of detecting differences between treatment means and research methodology issues in laboratory and field experiments. AP Express publishers limited: Nigeria, 2002.

D.M Musyimi, S.W Kahihu, D,K Buyela, and P.A Sikuku. “Allelopathic effects of Mexican sunflower [Tithonia diversifolia (Hemsl) A. Gray] on germination and growth of spiderplant (Cleome gynandra L.).” Journal of Biodiversity and Environmental Sciences, vol. 2, no.8, pp.:26-35, 2012.

R.O Oyerinde, O.O Otusanya, and O.B Akpor. “Allelopathic effect of Tithonia diversifolia on the germination, growth and chlorophyll contents of maize (Zea mays L.).” Scientific Research and Essay, vol. 4, no.12, pp. 1553-1558, 2009..

K.S Chukwuka, M.C. Obiakara and I.A. Ogunsumi. “Effects of aqueous plant extracts and inorganic fertilizer on the germination, growth and development of maize (Zea mays L.).” Journal of Agricultural Sciences ,vol. 59, no. 3, pp. 243-254, 2014.

D.R Batish, K. Lavanya, H.P Singh and R.K Kohli. . “Root-mediated allelopathic interference of nettle-leaved goosefoot (Chenopodium murale) on wheat (Triticum aestivum).” J. Agronomy and Crop Science, vol. 193, pp. 37–44, 2007..

M.R Asgharipour and M. Armin. “Inhibitory effects of Sorghum halepens root and leaf extracts on germination and early seedling growth of widely used medicinal plants.” Advances in Environmental Biology, vol. 4 no.2, 316-324.

S. Hussain, S. Siddiqui, S. Khalid, A. Jamal, A. Qayyum and Z. Ahmed. Z. “Allelopathic potential of Senna (Cassia angustifolia) on germination and seedling characters of some major cereal crop and their associated grassy weeds.” Pak. J. Bot. vol. 39, pp. 1145-1193, 2007.

M. Naseem, M. Aslam M, Ansar and M. Azhar. “Allelopathic effects of sunflower water extract on weed control and wheat productivity.” Pakistan Journal of Weed Science Research, vol. 15, no.1, pp. 107-116, 2009.

S.U Chon, Y. Kin and J.C. Kee. “Herbicidal potential and quantification of causative allelochemicals from several compositae weeds.” Weed Res., vol. 43, pp. 444-450, 2003.

U. Blum, R. Shafer and M.E Lemon. “Evidence for inhibitory allelopathic intraction including Phenolic acids in field soils: Concept vs. An experimental model.” Crit Rev. Plant Sci., vol. 18, pp. 673-693, 1999.

N.B Singh, S. Ranjana and R. Singh. “Effects of leaf leachate of Eucalyptus on germination, growth and metabolism of green gram, black gram and peanut.” Allelopathy J., vol. 11, pp. 43-52, 2003.

M.A Salam, . and H. Kato-Noguchi. “Allelopathic potential of methanol extract of Bangladesh rice seedlings. Asian J. Crop Sci., vol. 2, pp. 70-77, 2010.

N. Nishida, S. Tamotsu, N. Nagata, C. Saito and A. Sakai. “Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla: Inhibition of cell proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedlings.” J. Chem. Ecol., vol. 31, pp. 1187-1203, 2005.

L. Ziaebrahimi, R.A. Khavari-Nejad, H. Fahimi and T. Nejadsatari. “Effects of Aqueous Eucalyptus Extracts on Seed Germination, Seedling Growth and Activities of Peroxidase and Polyphenoloxidase in Three Wheat Cultivar Seedlings (Triticum aestivum L.).” Pakistan Journal of Biological Sciences, vol. 10, pp. 3415-3419, 2007.

C.H Chou. “Allelopathy in relation to agricultural productivity in Taiwan: Problem and prospects.” In Allelopathy-basic and applied aspects, (ed.). Rizvi SJH & V Rizvi. Chapman and Hall: London. 1992, pp. 179-200.

M.H.R Pramanik, T. Minesaki, T. Yamamoto Y., Matsui and H. Nakano. “Growth inhibitors in rice-straw extracts and their effects on Chinese vetch (Astragalus sinicus) seedlings.” Weed Biol. Manage, vol. 1, pp. 133-136, 2001.

S. Roy, M. Asaduzzaman, M.H.R. Pramanik and A. K. M. A. Prodhan. “Effect of banana plant extracts on germination and seedling growth of some vegetable crops.“ Bangladesh J. Crop Sci. vol. 17, no.1 ,pp. 235-242, 2006.

P. Pukclai and H. Kato-Noguchi. “Evaluation of Allelopathic Activity of Hibiscus sabdariffa L.” Advances in Biological Research, vol. 5, no.6, pp. 366-372, 2011.

F. A. Einhellig. “The Physiology of Allelochemical Action: Clues and Views.” In Allelopathy from Molecules to Ecosystems, Reigosa, M.J. and N. Pedrol Ed.. Science Publishers, Enfield: New Hampshire, 2002.

Downloads

Published

2017-12-06

How to Cite

M. Etukudo, M., & Egbe, A. (2017). A Study on Germination and Early Seedling Growth of Zea mays L.in Hibiscus sabdariffa Linn. Extract Treated Medium. International Journal of Sciences: Basic and Applied Research (IJSBAR), 36(8), 263–272. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/8551

Issue

Section

Articles