Bacterial Flora of Arachis Hypogea Plants from Agricultural Fields of Punjab, Pakistan
Keywords:
Rhizosphere, Bacteria, Arachis hypogeal, Metal Resistance, Antibiotics, Acid Phosphatase.Abstract
The study was carried out to identify the rhizo-microorganisms from the roots of Arachis hypogea plants collected from agricultural fields of upper Punjab (Chakwal), Pakistan. Eight purified bacterial isolates were further identified by 16S rRNA gene sequencing. Bacillus spp were found to be most abundant in the rhizophere of Arachis hypogea. The isolated bacteria were mostly mesophilic (grow at 37°C and 42°C) and resistant to chloramphenicol. All isolates were found to be resistant to metals such as Mn, Pb, Co and Cr (100 µg/ml). However, these were sensitive to Hg and Cd at all the concentrations studied (100, 500 & 1000 µg/ml). Almost all isolates were sensitive to all metals at higher concentrations except few isolates which were resistant to Zn. The plant growth promoting ability was tested for Arachis hypogea seeds in pot experiment. No nodule formation was observed with any of the isolates while all of them promoted the growth of Arachis hypogea.
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