The Effect of Adding Linalol to Formalin on the Effectiveness of Embalming of Gastrocnemius Muscle
Keywords:
Linalol, Formalin, Embalming, Gastronemius.Abstract
Formaldehyde has been a standardized embalming agent. However, it has a strong odor and potential adverse health effects. Linalool, a natural fragrance, has been shown to have an antibacterial activity. This study aimed to determine the effect of adding linalool to formaldehyde on the effectiveness of embalming rat gastrocnemius muscle. In this posttest only control group design, 25 Wistar rats were randomly divided into 5 groups. Forty eight hours postmortem, the first group injected with 10 ml formaldehyde was used as control, the remaining three groups were injected with 10 ml formaldehyde in combination with 2.5 ml, 5ml or 67.5 ml or 10 ml of linalool respectively. Forty eight hours post mortem, the histologic features of the gastrocnimeus muscle of rats was assessed. The data were tested for normality and homogeneity followed by Kruskal Wallis test. Mean number of post mortem autolytic cells of gastrocnimeus muscles for the four groups were 1.88±3.67, 10.58±11.70, 15.00±12.65, 16.31±27.31, 4.49±2.93 respectively. There was no significant difference (p>0.05). Adding linalool to formaldehyde has no effect on the effectiveness of embalming of rat gastrocnimeus muscle.
References
Zulham. Histoteknik dasar. 1st ed. Medan: Departemen Histologi 2009; FK USU
Edmund G, Brown JR. Information and instructions for embalmer licensure. 2011. [Accessed on 26 Januari 2016] Available from: http://www.cfb.ca.gov
Atmadja DS. Tatacara dan pelayanan pemeriksaan serta pengawetan jenazah pada kematian wajar. Jakarta: Bagian Kedokteran Forensik dan Medikolegal FKUI / RSUPN Cipto Mangunkosumo. 2002. [diakses pada 26 Januari 2017] diunduh dari: http://www.tatacaraembalming.com.
SIDS Initial Assessment Report, George Dr.. 11 February 2002; Switzerland
Coleman et al. An improved low-formaldehyde embalming fluid to preserve cadavers for anatomy teaching. Journal of anatomy.1998 : 443-446
Eells JT, Mc Martin KE, Black K, et al. Formaldehyde poisoning: Rapid metabolism to formic acid. JAMA 1981; 246: 1237-38
Abdollahi M, Hosseini A. Encyclopedia of toxicology Ed 3. Formaldehyde. 2014 : 653–656
Turan, et al. The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy. Jurnal Annals of Anatomy – Anatomischer Anzeiger 2016; 209: 11-17
Srinivasan K, Ramarao P. Animal models in type 2 diabetes research : an overview. Indian J Med Res 2007; 125 : 451-472
Murray, R., Granner, D., Rodwell, V., 2006, Harper’s Illustrated Biochemistry, 27th Edition, The McGraw-Hill Companies, United States of America, 207.
Kumar, V., Cotran, R.S., Robbins, S.L. Buku Ajar Patologi, Edisi 7, Volume 1, 2007; EGC, Jakarta, 189
Maksoud, Gomma Abel and El-Amin, Abdel Rahman. A Review On Material Used During The Mummification Process In Ancien Egypt. Mediterranean Archaeology and Archaeometry 2011; 11( 2) : 129-150
Brenner, E. Human body preservation – old and new techniques. Journal of Anatomy 2014; 224(3) : 316–344. http://doi.org/10.1111/joa.12160
Andrew J Connolly, Walter E. Finkbeiner, Philip C. Ursell, Richard L. Davis. Autopsy Pathology. Philadelphia : Elsevier. 2016: 26
Rowe RC, Sheske PJ, Quinn ME. 2009. Handbook of Pharmaceutical Excipients. Lexi-Comp: American Pharmaceutical Association, Inc. p. 418, 685.
Giroun C. Mariangela DC. Aurelie B., Vincent V., Nicolas CL., Bernard B. E-Cigarettes: A Review of New Trends in Cannabis Use. Int J Environ Res Public Health 2015;12 (8):9988-10008
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.
