The Effect of Adding Linalol to Formalin on the Effectiveness of Embalming of Gastrocnemius Muscle

  • Setyo Trisnadi Department of Forensic Science Legal Medicine, Faculty of Medicine, Universitas Islam Sultan Agung, Jln. Raya Kaligawe KM. 4 Semarang, 50112
  • Eni Widayati Department of Chemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Jln. Raya Kaligawe KM. 4 Semarang, 50112
  • Dymas Rachma Kumala Student of Faculty of Medicine, Faculty of Medicine, Universitas Islam Sultan Agung, Jln. Raya Kaligawe KM. 4 Semarang, 50112
Keywords: Linalol, Formalin, Embalming, Gastronemius.


Formaldehyde has been a standardized embalming agent. However, it has a strong odor and potential adverse health effects. Linalool, a natural fragrance, has been shown to have  an  antibacterial  activity. This study aimed to determine the effect of adding linalool to formaldehyde on the effectiveness of embalming rat gastrocnemius muscle. In  this  posttest  only control  group  design, 25 Wistar  rats  were randomly  divided  into 5  groups.  Forty eight hours postmortem, the first group injected with  10  ml  formaldehyde  was used as control, the remaining three groups  were injected  with 10 ml  formaldehyde in  combination  with 2.5 ml, 5ml or 67.5  ml  or  10  ml  of  linalool respectively. Forty eight hours post  mortem, the histologic features of the gastrocnimeus muscle of rats was assessed. The data were tested for normality and homogeneity followed by Kruskal Wallis test. Mean number of post mortem autolytic cells of gastrocnimeus muscles for the four groups were 1.88±3.67, 10.58±11.70, 15.00±12.65, 16.31±27.31, 4.49±2.93 respectively. There was no significant difference (p>0.05). Adding linalool to formaldehyde has no effect on the effectiveness of embalming of rat gastrocnimeus muscle.


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