Isolation and Molecular Identification of Lactic Acid Bacteria (Lab) from Nile Tilapia (Oreochromis niloticus) as Potential Pathogen Antagonist
Keywords:
antibacterial, human enteric pathogen, Lactic Acid Bacteria, Nile tilapia .Abstract
Five lactic acid bacterial isolates from the intestinal tract of Nile Tilapia (Oreochromis niloticus) were tested for their antagonistic activity against common human pathogens. Only three were identified using 16S rRNA gene sequencing as Pediococcus pentosaceus (isolates TI-8 and TI-11) and Enterococcus avium (TI-17) showing 99% homology. Among the isolates, TI-8 and T1-11 (P. pentosaceus) viable cell cultures were found to have the highest inhibitory activity against Escherichia coli ATCC 25922, surpassing that of the positive control, Tetracyline. Though showing significant inhibition also to Pseudomonas aeruginosa BIOTECH 1335, Staphylococcus aureus BIOTECH 1582, Salmonella enteriditis BIOTECH 1963, Salmonella typhimurium BIOTECH 1826, and Vibrio cholerae BIOTECH 1967, T1-17 (Enterococcus avium) is not effective against S. aureus BIOTECH 1582. On the other hand, T1-8 and T1-11 (P. pentosaceus) cell free supernatant cultures exhibited inhibitory activity against P. aeruginosa BIOTECH 1335, S. aureus BIOTECH 1582, S. enteriditis BIOTECH 1963, S. typhimurium BIOTECH 1826, and V. cholerae BIOTECH 1967.
The LAB isolate T1-17 (E. avium) exhibited an inhibitory activity against S. enteriditis BIOTECH and V. cholerae BIOTECH 1967. T1-11 (P. pentosaceus) was susceptible to Tetracycline and Amoxicillin; T1-8 (P. pentosaceus) was susceptible to Amoxicillin and Cephalexin; and T1-17 (E. avium) was susceptible to Amoxicillin.
References
Sharma, K. Textbook of Microbiology. Ane Books Pvt. Ltd. New Delhi, India; 2011. pp. 7-9;681-682.
Versalovic, J and Wilson, M. Therapeutic Microbiology: Probiotics and Related Strategies. ASM Press. American Society for Microbiology; 2008.
Al-Faragi, Jamal K.H. and Sundus A.A. Alsaphar. Isolation and identification of Bacillus subtilus as probiotic from intestinal microflora of common carp Cyprinus carpio L. Proceeding of the Eleventh Veterinary Scientific Conference 2012;355-361.
Hoves, H., Norgaard, H., & Mortensen, B.P. (1999). Lactic Acid Bacteria and Human Gastrointestinal Tract. Euro J Clin Nutr, 53, 339–350.
Harish, K. and Varghese, T. (2006). Probiotics in Human-Evidence based Review. Calicut Med J, 4(4), e3.
Heyman, M. (2000). Effects of Lactic Acid Bacteria on Diarrheal Disease. J Amer Coll Nutr, 19(2), 137S–146S.
Kinova, S.H., Bilkova, A., & Bukovsky, M. (2008). Lactobacilli and their Probiotic Properties. Ceska Slov Farm, 57(2), 95–8.
Schaafsma, G. (1993). Lactose intolerance and consumption of cultured dairy products — a review. IDF Nutrition Newsletter, 2, 15–16.
Rauta, Pradipta R., Dhupal, Madhusmita and Nayak, Bismita. 2013. Screening and Characterization of Potential Probiotic lactic acid bacteria isolated from vegetable waste and fish intestine. Int. J. of Current Microbiology and Applied Sciences. 2 (8): pp. 234-244.
Talpur, A.D., Memon, A.J., Khan, M.I., Ikhwanuddin M., Danish, Daniel M.M. and Abol-Munafi, A.B. (2011). Isolation and Screening of Lactic Acid Bacteria from the Gut of Blue Swimming Crab, P. pelagicus, an in vitro Inhibition Assay and Small Scale in vivo Model for Validation of Isolates as Probiotics. Journal of Fisheries and Aquatic Science.
Flores, Maurilio L. and Novoa, Miguel Olvera A. (2013). The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets. Lat. Am. J. Aquat. Res. 41(3): 490-497.
Lim, Char Yee, M.Y. Ina-Salwany and Yasser M. Abdelhadi. (2011). Antibacterial Ability and Molecular Characterization of Probionts Isolated from Gut Microflora of Cultured Red Tilapia. Asian Journal of Animal and Veterinary Advances, 8: 116-123.
Sonomoto, K. and Yokota, A. (2011). Lactic Acid Bacteria and Bifidobacteria: Current Progress in Advanced Research. Caister Academic Press. ISBN 978-1-904455-82-0.
Riley MA, Wertz JE. (2002). Bacteriocins: evolution, ecology, and application. Annu Rev Microbiol. 56:117–137. [PubMed: 12142491].
Smith, JL., Orugunty, R., Hillman, JD. (2007). Lactobiotic production by Streptococcus mutants: their uses in replacement therapy for the prevention of dental caries and as antibiotics for the treatment of various infectious diseases. In: Riley, MA.; Gillor, O., editors. Research and applications in bacteriocins. Horizon Bioscience. 95-115.
Gillor, O., Etzion, A. and Riley, M.A. (2008). The dual role of bacteriocins as anti- and probiotics. Appl Microbiol Biotechnol. 81(4): 591–606.
Altuntas, Gunes E. (2013). Bacteriocins: A natural way to combat with pathogens. FORMATEX. Retrieved on March 2, 2016. From: http://www.formatex.info/microbiology4/ vol2/1005-1015.pdf.
Myllyluoma, E., Kajander, K., Mikkola, H., Kyrönpalo, S., Rasmussen, M., Kankuri, E., Sipponen, P., Vapaatalo, H., & Korpela, R. (2007). Probiotic intervention decreases serum gastrin-17 in Helicobacter pylori infection. Digest Liver Dis, 39 (6), 516–523.
Lawalata, Helen Joan, Langkah Sembiring, and Endang Sutriswati Rahayu. (2011). Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, an Indonesian Traditional Fermented Fish Product. Indonesian Journal of Biotechnology. 16 (2),93-99.
CLSI. (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24. Wayne, PA: Clinical and Laboratory Standards Institute.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.
