Efficiency of Diode Laser as Root Canal Disinfectant Against Enterococcus faecalis: An in Vitro Study
Keywords:
Diode laser, Enterococcus faecalis.Abstract
The objective of the in vitro study is to determine the disinfection ability of diode laser 1064 nm at different powers and duration of times against E. faecalis and detect the optimal power and time of diode laser 1064nm. Eighty human extracted single-rooted teeth were decoronated to a length 14mm from the apical foramen to the cervical border of the root, then adjusted working length to 13 mm, autoclaved and inoculated with a suspension of E. faecalis at a concentration 4×105 cfu and incubated at 37?C for 24 hours. Sixty samples were irradiated at different powers and irradiation time 30 and 60 sec, the remainder 20 samples were control positive and negative groups. All powers and times for laser 1064 nm had significant antimicrobial effects against E. faecalis. Diode laser device seems to be highly suitable for killing E. faecalis if appropriate energy and irradiated time used.
References
Jain P, Yeluri R, Garg N, Mayall S, Rallan M, Gupta S, Pathivada L. A Comparative Evaluation of the Effectiveness of Three Different Irrigating Solution on Microorganisms in the Root Canal: An Invivo Study. J Clin Diagn Res. 2015;9(12):ZC39-42.
Sundqvist G. Ecology of the root canal flora. J Endod. 1992;18: 427-430.
Sundqvist G, Figdor D, Persson S, Sjögren U . Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol. 1998; 85(1): 86-93.
Peters OA, Laib A, Göhring TN, Barbakow F . Changes in root canal geometry after preparation assessed by high-resolution computed tomography. J Endod. 2001; 27:1-6.
Vianna ME, Gomes BP, Berber VB, Zaia AA, Ferraz CC, de Souza-Filho FJ . In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod. 2004; 97: 79-84.
Benedicenti S, Speicato A and Resch A . Creation laser: applications of laser light in root canal disinfection. Productsplus. 2008; 2: 10-12.
Estrela C, Bueno MR, Couto GS, Rabelo LE, Alencar AH, Silva RG, Pecora JD, Sousa-Neto MD. Study of Root Canal Anatomy in Human Permanent Teeth in A Subpopulation of Brazil's Center Region Using Cone-Beam Computed Tomography - Part 1. Braz Dent J. 2015; 26(5):530-6.
Walters M, Baumgartner J and Marshall J. Efficacy of irrigation with rotary instrumentation. J Endod. 2002; 28(12): 837–9.
Elnaghy AM, Elsaka SE. Effect of sodium hypochlorite and saline on cyclic fatigue resistance of WaveOne Gold and Reciproc reciprocating instruments. Int Endod J. 2017; 50(10):991-8.
Bach G. Use of laser systems in endodontics. Roots Int Magazin Endodontol. 2006; 1(1): 53- 57.
Recai Zan, Ihsan Hubbezoglu, Murat Unal. Evaluation of temperature rises during the application of different power levels of potassium titanyl phosphate and neodymium-doped:yttrium aluminum garnet lasers to external primary root canals. journal of dental sciences. 2016; Vol. 11, Issue 4, pp. 365-369
Schoop U, Kluger W, Dervisbegovic S, Goharkhay K, Wernisch J, Georgopoulos A, Sperr W and Moritz A. Innovative wavelengths in endodontic treatment. Lasers Surg Med. 2006; 38: 624–630.
Garcez AS, Núñez SC, Lage-Marques JL, Jorge AOC, and Ribeiro MS. Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102(4): 93-98.
Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A and Sperr W. Bactericidal effect of different laser systems in the deep layers of dentine. Lasers Surg Med. 2004; 35(2): 111-116.
Hendy J. Newest 1064nm semiconductor lasers effectiveness in periodontics and endodontics. In: Abstracts, 5th SOLA Laser Congress. Vienna, Austria. J Oral Laser Appl. 2009; 9(2/3): 154-155.
Meire MA, De Prijck K, Coenye T, Nelis HJ and De Moor RJ. Antimicrobial efficacy of Nd:YAG and Er:YAG irradiation on Enterococcus faecalis biofilms on dentine Disks. J Oral Laser Appl. 2009; 9(2/3): 164-156.
Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT and Matsumoto K. Effect of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed laser Surg. 2005; 23(6): 575-581.
Gordon W, Atabakhsh VA, Meza F, Doms A, Nissan R, Rizoiu I and Stevens RH. The antimicrobial efficacy of the Erbium, Chromium:Yttrium-Scandium-Gallium Garnet laser with radial emitting tips on root canal dentine walls infected with Enterococcus faecalis. J Am Dent Assoc. 2009; 138(7): 992-1002.
Ando Y, Aoki A, Watanabe H and Ishikawa I. Bactericidal effect of Erbium:YAG laser on periodontopathic bacteria. Lasers Surg Med. 1996; 19(2): 190–200.
Elmros T, Burman LG, Bloom GD. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1976; 126: 969–976.
Rosenberg B, Kemeny G, Switzer RC, Hamilton TC (1971): Quantitative evidence for protein denaturation as the cause of thermal death. Nature. 232(13): 471–473.
Parirokh M, Eghbal MJ, Asgary S, Ghoddusi J, Stowe S, Forghani F and Shahravan A. Effect of 808nm diode laser irradiation on root canal walls after smear layer removal: a scanning electron microscope study. Iran Endod J. 2007; 2(2): 37-46.
Moritz A, Jakolitsch S, Goharkhay K, Schoop U, Kluger W, Mallinger R, Sperr W and Georgopoulos A. Morphologic changes correlating to different sensitivities of Escherichia coli and Enterococcus faecalis to Nd:YAG laser irradiation through dentine. lasers surg. Med. 2000; 26(3): 250-261.
Gutknecht N, Franzen R, Schippers M, and Lampert F. Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg. 2004; 22(1): 9–13.
Lee BS, Lin YW, Chia JS, Hsieh TT, Chen MH, Lin CP and Lan WH. Bactericidal effects of diode laser on Streptococcus mutans after irradiation through different thickness of dentine. Lasers Surg Med. 2006; 38: 62–69.
Kreisler M, Kohnen W, Beck M, Al Haj H, Christoffers AB, Götz H, Duschner H, Jansen B and d’Hoedt B. Efficacy of NaOCl/H2O2 irrigation and GaAlAs laser in decontamination of root canals in vitro. Lasers Surg Med. 2003; 32: 189–196.
Moritz A, Gutknecht N, Gohrakhay K, Schoop U, Wernisch J, Sperr W. In vitro irradiation of infected root canals with a diode laser: results of microbiologic, infrared spectrometric, and stain penetration examinations. Quintessence Int. 1997, 28(3): 205-209.
Bergmans L, Moisiadis P, Teughels W, Van Meerbeek B, Quirynen M and Lambrechts P. Bactericidal effect of Nd:YAG laser irradiation on some endodontic pathogens ex vivo. Int Endod J. 2006; 39(7): 547–557.
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