Efficiency of Diode Laser as Root Canal Disinfectant Against Enterococcus faecalis: An in Vitro Study

  • Njwan F. Shehab Department of Conservative Dentistry, College of Dentistry, Mosul University, Mosul-Iraq
  • Nawfal A. Zakaria Department of Conservative Dentistry, College of Dentistry, Mosul University, Mosul-Iraq
  • Mahmoud Y. M. Taha Department of Dental Basic Science, College of Dentistry, Mosul University, Mosul-Iraq
Keywords: Diode laser, Enterococcus faecalis.


The objective of the in vitro study is to determine the disinfection ability of diode laser 1064 nm at different powers and duration of times against E. faecalis and detect the optimal power and time of  diode laser 1064nm. Eighty human extracted single-rooted teeth were decoronated to a length 14mm from the apical foramen to the cervical  border of the root, then adjusted working length to 13 mm, autoclaved and inoculated with a suspension of E. faecalis at a concentration 4×105 cfu and  incubated  at 37˚C for 24 hours.  Sixty  samples were irradiated at  different powers and irradiation time 30 and 60 sec, the remainder 20 samples were control positive and negative groups.  All powers and times for laser 1064 nm had significant antimicrobial effects against E. faecalis. Diode laser device seems to be highly suitable for killing E. faecalis  if appropriate energy and irradiated time used.


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