New Synthesized Derivatives from N-Substituted-4-Oxo-[1] Benzopyrano [4,3-c] Pyrazole Influenced Proliferation, Viability, Spreading and Invasion of Human Liver Tumor Cells

Authors

  • Hela Guesmi Laboratory of Genetic, Biodiversity, and Bioresources Valorisation LR11ES41, Higher Institute of Biotechnology of Monastir, Avenue Tahar Haddad (Bp 74), Monastir 5000, Tunisia
  • Oualid Haddad Inserm U1148, Laboratory for Vascular Translational Science, UFR SMBH, Paris 13 University, Sorbonne Paris Cité, Groupe Biothérapies et Glycoconjugués, Bobigny, 93000, France
  • Kevin Bassand Inserm U1148, Laboratory for Vascular Translational Science, UFR SMBH, Paris 13 University, Sorbonne Paris Cité, Groupe Biothérapies et Glycoconjugués, Bobigny, 93000, France
  • Rafik Gharbi Laboratory of Heterocyclic Synthesis and Chemistry of Natural Compounds LR11ES39, Faculty of Sciences of Monastir, University of Monastir, route de Kairouan, 5000 Monastir, Tunisia
  • Moncef Msadek Laboratory of Heterocyclic Synthesis and Chemistry of Natural Compounds LR11ES39, Faculty of Sciences of Monastir, University of Monastir, route de Kairouan, 5000 Monastir, Tunisia
  • Frederic Chaubet INSERM U1148, Laboratory for VascularTranslational Science (LVTS), Institut Galilée, Paris 13 University of, Sorbonne Paris Cité, 99 Avenue Jean-Baptiste Clément, Villetaneuse, France
  • Marc Lecouvey CSPBAT, UMR 7244 CNRS, Paris 13 University, 93017 Bobigny, France
  • Raoui Mounir Maaroufi Laboratory of Genetic, Biodiversity, and Bioresources Valorisation LR11ES41, Higher Institute of Biotechnology of Monastir, Avenue Tahar Haddad (Bp 74), Monastir 5000, Tunisia
  • Hanna Hlawaty Inserm U1148, Laboratory for Vascular Translational Science, UFR SMBH, Paris 13 University, Sorbonne Paris Cité, Groupe Biothérapies et Glycoconjugués, Bobigny, 93000, France

Keywords:

Fraud pentagon, fraudulent financial reporting, non-financial company.

Abstract

Background/Aim: There is an unsatisfied clinical demand to develop new anticancer agents. The aim of the current study was to synthesize new coumarin derivatives using two different synthetic methodologies and to evaluate their anticancer activity. Materials and methods: Four coumarin derivatives were synthesized and evaluated for their anticancer activities. The structures of all compounds were confirmed by infrared (IR), UV-vis, Nuclear magnetic resonance (NMR) 13C NMR, 1H NMR, and high-resolution mass spectrometry (HRMS) analysis.

All the synthesized compounds (4, 5, 8 and 9) were analyzed for their anti-proliferative (MTT and LDH assays and cell cycle studied with flow cytometry) and anti-invasive activity (spreading and invasion tests) on human hepatoma cell lines Huh-7 in vitro. Doxorubicin was used as control in order to compare their anti-tumoral effects. Results. All the synthesized compounds are potential inhibitors of proliferation, viability, spreading and invasion of human liver tumor cells with a 50% inhibitory Concentration range,  IC50=10.37 ?M to 12.94 ?M. Conclusion. This study could lead to the identification of a new target therapy for human Hepatocellular carcinoma (HCC) or other cancers.

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Published

2018-12-30

How to Cite

Guesmi, H., Haddad, O., Bassand, K., Gharbi, R., Msadek, M., Chaubet, F., Lecouvey, M., Mounir Maaroufi, R., & Hlawaty, H. (2018). New Synthesized Derivatives from N-Substituted-4-Oxo-[1] Benzopyrano [4,3-c] Pyrazole Influenced Proliferation, Viability, Spreading and Invasion of Human Liver Tumor Cells. International Journal of Sciences: Basic and Applied Research (IJSBAR), 42(5), 167–185. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/9648

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Vol. 42 No. 5 (2018)

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Articles

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