Study of the Effect of Chemotherapy on Some Serum Antioxidants and Partly Purified Glutathione Peroxidase from Patients with Breast Cancer
Keywords:
Breast cancer, Glutathione peroxidase, Glutathione, Catalase, Uric acid, Purification of glutathione peroxidase, chemotherapyAbstract
Cancer is considered one of the most serious diseases that threaten the society at present. Accordingly, some antioxidants have been studied about breast cancer during chemotherapy and the effect of this treatment on antioxidants that protect cells from oxidation and damage associated with cancer. This study was conducted on (92) Participants; (52) of which for patients with breast cancer who are aged between (25-80) year. Additionally, (40) healthy people aged between (22-75) year were enrolled. The samples were obtained from the Center for Oncology in Azadi Teaching Hospital in Kirkuk. This research deals with the study of glutathione peroxidase (GPx) using gel filtration (Sephadex-50) and the dimension column (28×1.5) cm. The serum from a patient with breast cancer was partially purified and compared with that from healthy female control. The effect of chemotherapy on GPx, Catalase (CAT), Glutathione (GSH) and Uric acid (U. A) has studied as an antioxidant. The results showed, A substantial decrease in the catalase enzyme in the different groups of chemical doses (A, B, C and D) Group (A) consists of patients undergoing chemotherapy from (1-3) Chemotherapy doses. Group (B) consists from (4-6) Chemotherapy doses, Group (C) consists from (7-9) Chemotherapy doses, Group (D) consists from (10-12) Chemotherapy doses at a Statistical level (p ?0.01). On the other hand, the group (E) consists from (13-16) Chemotherapy doses has found no considerable difference, the drugs that make up the chemotherapy given to patients (Adriamycin, Cytoxan, methotrexate, Fluorouracil). the level of enzyme activity (GPx) at a Statistical level (p ?0.01). (C, D and E) groups is decrease, either groups represented by (A and B) with no significant difference was recorded, the level of enzyme activity (GSH) at a Statistical level (p ?0.01).
(A) group is decrease, either groups represented by (B,C,D,E) groups with no significant difference was recorded,.Lastly, in the Uric acid, there are No significant differences in groups that differ in chemical doses.
References
. Rowland, J. H. (2018)," Cancer survivorship. Advancing the Science of Implementation across the Cancer Continuum" 220.
. Kleckner, N. E.; Chatzi, K.; White.S.; M. A.; Fisher.; J. K.; & Stouf, M. (2018)," Coordination of Growth, Chromosome Replication/Segregation, and Cell Division in E. coli" Frontiers in microbiology, 9.
. Köller, M.; Ziegler, N.; Sengstock C.; Schildhauer, T. A.; & Ludwig, A, (2018)," Bacterial cell division is involved in the damage of gram-negative bacteria on a nano-pillar titanium surface" Biomedical Physics & Engineering Express, 4(5), 055002.
. Davies, C.; Pan, H.; Godwin, J.; Gray, R.; Arriagada, R.; Raina, V., ... & Bradbury, J. (2013), "Long-term effects of continuing adjuvant tamoxifen to 10 years versus stopping at 5 years after diagnosis of oestrogen receptor-positive breast cancer: ATLAS, a randomised trial" The Lancet, 381(9869), 805-816.
. Gray, R. G.; Rea, D.; Handley, K.; Bowden, S. J.; Perry, P.; Earl, H. M., ... & Fernando, I. N. (2013)," aTTom: Long-term effects of continuing adjuvant tamoxifen to 10 years versus stopping at 5 years in 6,953 women with early breast cancer"
. Ramião, N.; Zanata, F.; Fortoul, T.; Nahas, F. X.; Ferreira, L. M.; Martins, P., ... & Santos, D. C. (2018), "Breast Implants: Far Beyond Just Aesthetic Surgery" In Women's Health and Biomechanics (pp. 83-94). Springer, Cham..
. Colaris, M.J., de Boer, M., van der Hulst, R.R. and Tervaert, J.W.C., 2017. Two hundreds cases of ASIA syndrome following silicone implants: a comparative study of 30 years and a review of current literature. Immunologic research, 65(1): 120-128.
. Insua-Rodríguez, J.; Pein, M., Hongu, T.; Meier, J.; Sinn, H. P.; Spaich, S., ... & Oskarsson, T. (2018)," 28 Stress signalling in breast cancer cells induces matrix components of stem cell niches that promote chemotherapy-resistant metastasis".
. Usiskin, I. M.; Li, F.; Irwin, M. L.; Cartmel, B.;& Sanft, T. B. (2018), "Association between lifestyle factors and completion of neoadjuvant chemotherapy for breast cancer".
. Rowland, J. C.; Hershman, D. L.; Kalinsky, K.; Crew, K. D.;Cheung, Y. K., & Lee, S. M. (2017)," Differences in global toxicity burden among patients and care providers during initial chemotherapy for breast cancer".
. Usiskin, I. M.; Li, F.; Irwin, M. L.; Cartmel, B., & Sanft, T. B. (2018), "Association between lifestyle factors and completion of neoadjuvant chemotherapy for breast cancer".
. Anampa, J.; Makower, D., & Sparano, J. A. (2015)," Progress in adjuvant chemotherapy for breast cancer: an overview" BMC medicine, 13(1), 195. Sipe Jr, H.J., Jordan, S.J., Hanna, P.M. and Mason, R.P., 1994. The metabolism of 17β-estradiol by lactoperoxidase: a possible source of oxidative stress in breast cancer. Carcinogenesis, 15(11): 2637-2643.
. Sáez-Freire, M. D. M.; Blanco-Gómez, A.;Castillo-Lluva, S.; Gómez-Vecino, A.; Galvis-Jiménez, J. M.; Martín-Seisdedos, C.; ... & Patino-Alonso, M. C. (2018)," The biological age linked to oxidative stress modifies breast cancer aggressiveness"
. Yang, H.; Jenni, S.; Colovic, M.; Merkens, H.; Poleschuk, C.; Rodrigo, I., ... & Webster, J. M. (2017)," 18F-5-Fluoroaminosuberic acid as a potential tracer to gauge oxidative stress in breast cancer models" Journal of Nuclear Medicine, 58(3), 367.
. Sakallı Çetin, E.; Nazıroğlu, M.; Çiğ, B.; Övey, İ. S.; & Aslan Koşar, P. (2017)," Selenium potentiates the anticancer effect of cisplatin against oxidative stress and calcium ion signaling-induced intracellular toxicity in MCF-7 breast cancer cells: involvement of the TRPV1 channel" Journal of Receptors and Signal Transduction, 37(1), 84-93.
. Sciegienka, S. J.;Solst, S. R.; Falls, K. C.;Schoenfeld, J. D.; Klinger, A. R., Ross, N. L.; ... & Fath, M. A. (2017)," D-penicillamine combined with inhibitors of hydroperoxide metabolism enhances lung and breast cancer cell responses to radiation and carboplatin via H2O2-mediated oxidative stress " Free Radical Biology and Medicine, 108, 354-361.
. Farhat, Z.; Browne, R. W.;Bonner, M. R.; Tian, L.; Deng, F.; Swanson, M.; & Mu, L. (2018)," How do glutathione antioxidant enzymes and total antioxidant status respond to air pollution exposure?. Environment international, 112, 287-293"
. Ilham, I.; Hapsari, F.; & Fotedar, R. (2018)," Growth, enzymatic glutathione peroxidase activity and biochemical status of juvenile barramundi (Lates calcarifer) fed dietary fermented lupin meal supplemented with organic selenium " Aquaculture Research, 49(1), 151-164.
. Grossmann, A., & Wendel, A. (2018)," PREPARATION OF GLUTATHIONE PEROXIDASE FROM BOVINE" Handbook Methods For Oxygen Radical Research, 39.
. Benhar, M. (2018)," Roles of mammalian glutathione peroxidase and thioredoxin reductase enzymes in the cellular response to nitrosative stress" Free Radical Biology and Medicine.
. Borchert, A.; Kalms, J.; Roth, S. R.; Rademacher, M.; Schmidt, A.; Holzhutter, H. G., ... & Scheerer, P. (2018)," Crystal structure and functional characterization of selenocysteine-containing glutathione peroxidase 4 suggests an alternative mechanism of peroxide reduction. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids"
. Alexander, N. M., & Jennings, J. F. (1974)," Radioimmunoassay of serum triiodothyronine on small, reusable Sephadex columns." Clinical chemistry, 20(10), 1353-1361.
. Nadar, S. S., & Rathod, V. K. (2016)," Magnetic macromolecular cross linked enzyme aggregates (CLEAs) of glucoamylase." Enzyme and microbial technology, 83, 78-87.
. Park, S. H.; Han, J. H.; Jeong, M. R.; Chon, J. W., & Song, Y. J. (2004)," The effects of uncooked powdered food on nutrient intake, body fat and serum lipid compositions in hyperlipidemic patients." Asia Pacific Journal of Clinical Nutrition, 13.
. Gaetani, G. F.; Kirkman, H. N.; Mangerini, R., & Ferraris, A. M. (1994)," Importance of catalase in the disposal of hydrogen peroxide within human erythrocytes." Blood, 84(1), 325-330.
. Green, M.J. and Hill, H.A.O. Chemistry of dioxygen. Methods Enzymol. 1984; 105:15
. Sedlak, J. and Lindsay, R. H. “Analytical biochemistry” 1968 p.192 . cited by Al-Zamely(2001). Al-Zamely, O. M.; Al-Nimer, M. S. and Muslih, R. K. Detection the level of peroxynitrite and related with antioxidants status in the serum of patient with acute myocardial infraction . Natl. J. Chem .2001; 4: 625-637.
. Chaney, A. L.; & Marbach, E. P. (1962)," Modified reagents for determination of urea and ammonia" Clinical chemistry, 8(2), 130-132.
. Aitchison, J. D., & Rachubinski, R. A. (1990)," In vivo import of Candida tropicalis hydratase-dehydrogenase-epimerase into peroxisomes of Candida albicans" Current genetics, 17(6), 481-486.
. Hegedűs, C.;Kovács, K.; Polgár, Z.; Regdon, Z.; Szabó, É., Robaszkiewicz, A., ... & Virág, L. (2018)," Redox control of cancer cell destruction. Redox biology."
. Karjalainen, E., & Repasky, G. A. (2016)," Molecular Changes During Acute Myeloid Leukemia (AML) Evolution and Identification of Novel Treatment Strategies Through Molecular Stratification. In Progress in molecular biology and translational science (Vol. 144, pp. 383-436)." Academic Press.
. Benfeitas, R.; Uhlen, M.; Nielsen, J., & Mardinoglu, A. (2017)," New challenges to study heterogeneity in cancer redox metabolism." Frontiers in cell and developmental biology, 5, 65.
. Chang, A. (2011)," Chemotherapy, chemoresistance and the changing treatment landscape for NSCLC." Lung cancer, 71(1), 3-10.
. Ko, J. H.; Gu, W.; Lim, I.; Bang, H.; Ko, E. A., & Zhou, T. (2014), "Ion channel gene expression in lung adenocarcinoma: potential role in prognosis and diagnosis." PloS one, 9(1), e86569.
. Valko, M.; Rhodes, C.; Moncol, J.; Izakovic, M. M., & Mazur, M. (2006)," Free radicals, metals and antioxidants in oxidative stress-induced cancer." Chemico-biological interactions, 160(1), 1-40.
. Visconti, R., & Grieco, D. (2009)," New insights on oxidative stress in cancer." Current opinion in drug discovery & development, 12(2), 240-245.
. Taysi, S.; Cikman, O.; Kaya, A.; Demircan, B.; Gumustekin, K.; Yilmaz, A., ... & Turkeli, M. (2008)," Increased oxidant stress and decreased antioxidant status in erythrocytes of rats fed with zinc-deficient diet." Biological trace element research, 123(1-3), 161-167.
. Moutet, M.; d’Alessio, P.; Malette, P.; Devaux, V., & Chaudière, J. (1998)," Glutathione peroxidase mimics prevent TNFα-and neutrophil-induced endothelial alterations." Free Radical Biology and Medicine, 25(3), 270-281.
. Jayasurya, A., Bay, B.H., Yap, W.M. and Tan, N.G., 2000. Correlation of metallothionein expression with apoptosis in nasopharyngeal carcinoma. British Journal of Cancer, 82(6): 1198-1198.
. Zhang, S.L., Mao, N.F., Sun, J.Y., Shi, Z.C., Wang, B. and Sun, Y.J., 2012. Predictive potential of glutathione S-transferase polymorphisms for prognosis of osteosarcoma patients on chemotherapy. Asian Pacific Journal of Cancer Prevention, 13(6): 2705-2709.
. Ge, J., Tian, A.X., Wang, Q.S., Kong, P.Z., Yu, Y., Li, X.Q., Cao, X.C. and Feng, Y.M., 2013. The GSTP1 105Val allele increases breast cancer risk and aggressiveness but enhances response to cyclophosphamide chemotherapy in North China. PloS One, 8(6): e67589- e67589.
. Blázquez, E., Velázquez, E., Hurtado-Carneiro, V. and Ruiz-Albusac, J.M., 2014. Insulin in the brain: its pathophysiological implications for States related with central insulin resistance, type 2 diabetes and Alzheimer’s disease. Frontiers in Endocrinology, 5: 161- 161.
. Liu, J., Xu, C., Ying, L., Zang, S., Zhuang, Z., Lv, H., Yang, W., Luo, Y., Ma, X., Wang, L. and Xun, Y., 2017. Relationship of serum uric acid level with non‐alcoholic fatty liver disease and its inflammation progression in non‐obese adults. Hepatology Research, 47(3): E104-E112.
. Sabeh, F.; Wright, T., & Norton, S. J. (1993)," Purification and characterization of a glutathione peroxidase from the Aloe vera plant." Enzyme and Protein, 47, 92-98.
. Gromer, S.; Arscott, L. D.; Williams, C. H.; Schirmer, R. H., & Becker, K. (1998)," Human placenta thioredoxin reductase isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds." Journal of Biological Chemistry, 273(32), 20096-20101
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.
