A Comparative Studies on Antimicrobial Activity and Antioxidant Activity of Different Extracts of Green Tea Camellia Sinensis (L.) Kuntze Leaf from Southern Shan State ( Myanmar )

Abstract

Green tea has many well-known effects and benefits. It has anti-diabetic, hypocholesterolemic, anti-inflammatory, anti-carcinogenic, anti-cavity, thermogenic, probiotic, antimicrobial and antiviral properties. In this research work, Green Tea  Camellia sinensis (L.) Kuntze leaf was collected from Panglong area, southern Shan state ,Myanmar. In the present research work, according to the preliminary phytochemical investigation, alkaloids, phenolic compounds, flavonoid, saponin, glycoside, tannins, reducing sugar, carbohydrate and ?-amino acid were found to be present in green tea. The yield percentage (%) of green tea  different  extracts of H₂O,  MeOH, EtOH and pet ether were  40, 15, 10 and 8  respectively .The vitro antimicrobial activity of different  extracts (EtOH , MeOH and H₂O) of green tea was screened by  Agar well diffusion method. In the present work, the tested microorganisms were Bacillus sublitis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus,Candida albicans and Escherichia coli. The antimicrobial activity against six microorganisms with the inhibition zone diameter ranged between 15 mm – 20 mm. The Antioxidant activity were   determined by DPPH assay method. IC₅₀ value of MeOH, EtOH and H₂O extracts of green tea were 13.57 µgmL^-1, 7.10 µgmL^-1  and 5.21 µgmL^-1  respectively.