New Cyclotides Isolated from the Roots of Allexix batangae (Violaceae)

Authors

  • Ernestine Nkwengoua Tchouboun Zondegoumb Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Cameroon
  • Olivier Ndogo Eteme Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Cameroon
  • Celine Nguefeu Nkenfou Higher Training Teaching School of Yaounde, Molecular Biology Center of Yaounde, Cameroon Chantal Biya International Reference Centre
  • David Setchaba Kanye Rhodes University, South-Africa
  • Barthélémy Nyasse Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Cameroon

Keywords:

Cyclotides, NMR, Violaceae, Allexix batangeae, Alba1, Alba2, Alba3, LC-MS, RP-HPLC and MALDI-TOF/MS, Sequence.

Abstract

Cyclotides are a very abundant class of plant peptides that display signi?cant sequence variability around a conserved cystine-knot motif and a head-to-tail cyclized backbone conferring them with remarkable stability. Their intrinsic bioactivities combined with tools of peptide engineering make cyclotides an interesting template for the design of novel agrochemicals and pharmaceuticals. In this work, we extend the knowledge about their sequence diversity by analysing the cyclotide content of a Violaceae specie native to Cameroon. Using an experimental approach, which was named sequence fragment assembly by MALDI-TOF/TOF, it was possible to characterize 4 cyclotides from the roots of Allexis batangeae. Amino acid sequencing of various enzymatic digests of cyclotides allowed the accurate assembly and alignment of smaller fragments to elucidate their primary structure.

References

. D.J. Craik, N.L. Daly, T. Bond, C. Waine. Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif, J. Mol. Biol., 1999, Vol. 294, pp. 1327-1336.

. J. Weidmann and D.J. Craik. Discovery, structure, function, and applications of cyclotides: circular proteins from plants. Journal of Experimental Botany, 2016, Vol. 67(16), pp. 4801–4812. doi:10.1093/jxb/erw210.

.G. Poth, J.S. Mylne, J. Grassl, R.E. Lyons, A.H. Millar, M.L. Colgrave, D.J. Craik,.Cyclotides Associate with Leaf Vasculature and Are the Products of a Novel Precursor in Petunia (Solanaceae), J. Biol. Chem., 2012, Vol. 287, pp. 27033-27046.

a]. A A.G. Poth, M.L. Colgrave, R.E. Lyons, N.L. Daly, D.J. Craik. Discovery of an unusual biosynthetic origin for circular proteins in legumes, Proc. Natl. Acad. Sci., 2011, Vol. 108, pp. 10127-10132.

b]. A.G. Poth, M.L. Colgrave, R. Philip, B. Kerenga, N.L. Daly, M.A. Anderson, D.J. Craik. Discovery of cyclotides in the Fabaceae plant family provides new insights into the cyclization, evolution, and distribution of circular proteins, ACS Chem. Biol., 2011, Vol. 6, pp. 345-355.

R. Hellinger; J. Koehbach; D. E. Soltis; E. J. Carpenter; G. K. Wong; and C. W. Gruber. Peptidomics of circular cysteine-rich plant peptides: analysis of the diversity of cyclotides from Viola tricolor by transcriptome and proteome mining. J. Proteome Res. 2015b, Vol. 14, pp. 4851–4862.

G. Achoundong. The African genus Allexis (Violaceae). A synoptic revision, National Herbarium Cameroon, 2010, 9p.

R. Burman, M.Y. Yeshak; S. Larsson; D.J. Craik; K.J. Rosengren and U. Göransson. Distribution of circular proteins in plants: large-scale mapping of cyclotides in the Violaceae. Front. Plant Sci. 2015, 6:855. doi: 10.3389/fpls.2015.00855.

A. Dickey and R. Faller. Examining the Contributions of Lipid Shape and Headgroup Charge on Bilayer Behavior. Biophysical Journal, 2008, Vol. 95, 2636–2646.

K. A. Babu and B. Jaykar. Development and Validation of Reverse phase high performance liquid chromatography method for simultaneous estimation of Paracetamol and Nabumetone in tablet dosage form Der Pharmacia Sinica, 2011, Vol. 2(5), pp. 192-197.

C. K. Wang; H.P. Wacklin and D. J. Craik. Cyclotides insert into lipid bilayers to form membrane pores and destabilize the membrane through hydrophobic and phosphoethanolamine-specific interactions. Chem., 2012, Vol. 2012, pp. 1-28. DOI 10.1074/jbc.M112.372011.

S. T. Henriques; Y.-H. Huang; M. A. R. B. Castanho; L. A. Bagatolli; S. Sonza; G. Tachedjian; N. L. Daly and D. J. Craik. Phosphatidylethanolamine Binding Is a Conserved Feature of Cyclotide-Membrane Interactions. The Journal of Biological Chemistry 2012, vol. 287(40), Pp. 33629-33643.

M. Fahradpour; P. Keov; C. Tognola; E. Perez-Santamarina; P.J. McCormick; A. Ghassempour and C. W. Gruber. Cyclotides Isolated from an Ipecac Root Extract Antagonize the Corticotropin Releasing Factor Type 1 Receptor. Front. Pharmacol., 2017, vol. 8, pp. 1-14. doi: 10.3389/fphar.2017.00616.

S. Zhu; M.-A. Sani; F. Separovic. Interaction of cationic antimicrobial peptides Australian de frogs with lipidic membranes. Peptide Science, 2018, vol. 110 (3), e24061.

L. D. Daleke and W. H. Huestis. Erytrocyte Morphology Reflects the transbilayer distribution of incorporation phospholipids. jcb.nopress.org , 1989, 1375-1385 ( 16 March 2019 )

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Published

2019-04-04

How to Cite

Zondegoumb, E. N. T., Ndogo Eteme, O., Nguefeu Nkenfou, C., Setchaba Kanye, D., & Nyasse, B. (2019). New Cyclotides Isolated from the Roots of Allexix batangae (Violaceae). International Journal of Sciences: Basic and Applied Research (IJSBAR), 45(1), 154–174. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/9893

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Vol. 45 No. 1 (2019)

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Articles

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