@article{Hathaichanoke Boonyarit_Sinkhan_Manaso_2022, title={Sensitivity and Heterozygous Balance of Globalfiler IQCTM PCR Amplification and Verifiler PlusTM Kits}, volume={61}, url={https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/13546}, abstractNote={<p>The Combined DNA Index System (CODIS) core loci was expanded from the existing 13 to 20 STRs, meaning a new kit will needed for many laboratories. A validation study was performed in accordance with SGWDAM guidelines Scientific Working Group on DNA. This main objective of this study was to measure the sensitivity and the heterozygous allele balance of Globalfiler IQC<sup>TM</sup> kit and Verifiler Plus<sup>TM</sup> kit in order to ensure that the kits would produce reliable profile. DNA samples ranging from 4 ng to 0.016 ng were amplified in 25 ul reaction volume to define the sensitivity and heterozygous balance of two systems. The data suggested that the Verifiler Plus<sup>TM</sup> kit is more sensitive than the Globalfiler IQC<sup>TM</sup> kit. However, the data of heterozygous balance also show that the Globalfiler IQC<sup>TM</sup> kit appeared to be more balanced peak heights than the Verifiler Plus<sup>TM</sup> kit. This performance demonstrated that both amplification kit can produce robust and reliable results in forensic DNA identification.  </p>}, number={1}, journal={International Journal of Sciences: Basic and Applied Research (IJSBAR)}, author={Hathaichanoke Boonyarit and Sinkhan, Nonglak and Manaso, Jakkapong}, year={2022}, month={Jan.}, pages={155–169} }