TY - JOUR AU - Hilya Miftahul Uswa, AU - Herry Sonjaya, AU - Gustina, Sri AU - Hasbi Hasbi, PY - 2022/10/04 Y2 - 2024/03/28 TI - The Concentration of Hydrogen Peroxide, Percentage of Motility, Viability, Plasma Membrane Integrity, and Abnormality of Frozen Semen of Horned and Polled Bali Bulls JF - International Journal of Sciences: Basic and Applied Research (IJSBAR) JA - IJSBAR VL - 64 IS - 1 SE - Articles DO - UR - https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/14584 SP - 104-115 AB - <p>The aim of the study was to determine the frozen semen quality of horned and hornless (polled) Bali bull including hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) concentration, motility, viability, plasma membrane integrity, and abnormalities. This study used frozen semen from 2 horned and 2 polled Bali bulls aged 5 to 7 years. The concentration of H<sub>2</sub>O<sub>2 </sub>was measured using 2-7-Dichlorodihydrofluorescein Diacetate (DCHF-DA) dye, motility was assessed subjectively under a microscope, viability was assessed by eosin-negrosin staining, plasma membrane integrity was calculated using the Hypoosmotic Swelling Test (HOS-Test), and abnormalities were evaluated with Sperm Stein Ready to USE stain.</p><p>The results showed that there was no significant difference (P&gt;0.05) between horned and polled based on the H<sub>2</sub>O<sub>2 </sub>concentration (2.37 ± 0.64 vs 2.65 ± 0.94), motility was (47.25 ± 5.04 vs 44.25 ± 8.12), plasma membrane integrity (50.62 ± 6.27 vs 52.75 ± 5.76), and abnormalities (15.90 ± 5.70 vs. 19.18 ± 6.39) but there was a significant difference (P&lt;0.05) in the viability value (53.71 ± 8.56 vs. 64.09 ± 9.48). It can be concluded that the quality of frozen semen of horned and hornless (polled) Bali cattle based on H<sub>2</sub>O<sub>2</sub> concentration, motility, plasma membrane integrity and abnormalities had the same conditions. The viability of spermatozoa from frozen semen of polled was better than that of horned Bali cattle.</p> ER -