Determination of Extracellular Enzymatic Activities of Bacteria Isolated from Insuyu Cave
Keywords:
Cave, microorganism, extracellular enzymesAbstract
Caves are often regarded as extreme environments due to their high humidity, stable low temperatures, limited nutrient availability, and minimal energy input. These unique characteristics attract researchers from various disciplines, including biology, chemistry, geology, and astronomy. Despite the presence of approximately 40,000 caves in Türkiye, microbiological studies in these habitats remain limited. One significant area of research involves the discovery of antimicrobial compounds and enzymes produced by microorganisms in isolated cave environments, driven by interspecies competition. In this study, bacterial strains were isolated from Insuyu Cave to evaluate their extracellular enzymatic activities. Seventy-five isolates were screened for lipase, protease, amylase, catalase, gelatinase, and urease production. Results showed that 39% of the strains exhibited lipolytic activity, 36% protease activity, 36% amylase activity, 91% catalase activity, 13% gelatinase activity, and 45% urease activity. The findings highlight the significant potential of caves as natural resources for industrial enzyme research.
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