Estimation of TNF- α and LDH in Chronic Periodontitis Patients in Mosul
AbstractThe objective of the study is to estimate salivary concentration of TNF-α and LDH level in chronic periodontitis and control group, and correlate them with periodontal parameters. The study group consisted of 44 patients suffering from chronic periodontitis, aging between ≤16-45 years old and 40 control samples collected from healthy individuals ranged between 16-40 years old .Chronic periodontitis was assessed on the basis of several periodontal parameters, including probing pocket depth (PPD), clinical attachment loss (CAL), bleeding on probing (BOP) and plaque index (PI). 5ml of unstimulated saliva was collected from patients and control groups to measure salivary biomarkers by ELISA technique. the mean concentrations of TNF-α in the study and control groups were (6.9pg/ml) and (5.6 pg/ml) respectively, data analysis showed no significant difference between the two groups (P< 0.05), and the mean LDH level in chronic periodontitis was (7.7855) and in control group the mean was (0.5555) and the difference was highly significant )p≤ 0.000). Salivary TNF-α and LDH showed positive correlation with clinical periodontal parameters.
Newman MG, Takei H, Klokkevold PR, Carranza FA. Caranzza’sClinical Periodontology.12th ed. Elsevier, 2014. pp. 700-890.
Lepilahti, JM., Ahonen, M-M., Hernandez, M., Munjal, S., Netuschil, L., and Uitto, VJ. Oral rinse MMP-8 point of care immune test identifies patents with strong periodontal inflammatory burden. Oral Dis. 17: 115-122. 2011.
Armitage, G. C., and Cullinan, MP. Comparison of the clinical features of chronic and aggressive periodontitis. Periodontol 2000. 53: 12-27.2010.
Newman, GM., Takei, HN., Carranza, FA., and EDS. Carranza Clinical Periodontology. Philidalphia. 12th ed. 2015, pp 96-112.
Almoharib, HS., AlMubarak, A., AlRowis, R., Geevarghese, A., Preethanath, RS., and Anil, S. Oral fluid based biomarkers in periodontal disease: part 1. Saliva. J Int Oral Health. . 6(4): 95-103. 2014.
Alonso,V., De La Pena,De. La., Diz Dios, P., and Tojo Sierra, R. “Relationship between lactate dehydrogenase activity in saliva and oral health status,” Arch Oral Biol. 52(10) : 911–915. 2007.
De La Pen, VA., Dios, DP., and Sierra, RT. Relationship between lactate dehydrogenase activity in saliva and oral health status. Arch Oral Biol. 52: 911-915. 2007.
Nomura, Y., Shimada, Y., Hanada, N., Numabe, Y., Kamoi, K., Sato, T. Salivary biomarkers for predicting the progression of chronic periodontitis. Arch Oral Biol. 57(4):413–420. 2012.
olwaczny ,M., Glas, J., and Torok, HP. Lack of association between the TNF-α G-308 A promoter polymorphism and periodontal disease. J Clin Periodontol. 31(6): 449-453. 2004.
Nikolopoulos GK, Dimou NL, Hamorakas SJ and Bagos PG. Cytokine gene polymorphism in periodontal disease: A metaanalysis of 53 studies including 4178 cases and 4590 controls. J Clin Periodontol. 35(9): 754-767. 2008
Aurer A, Jorgić-Srdjak K, Plančak D, Stavljenić-Rukavina A, and Aurer-Koželj J. Pro inflammatory factors in saliva as possible markers for periodontal disease. Coll Antropol. 29(2) 435-439. 2005.
Khongkhunthian, S; Kongtawelert, P; Ongchai, S; Pothacharoen, P Sastraruji, T; Jotikasthria, D and Krisanaprakornkit, S. Comparison between Two Biochemical Markers in Evaluating Periodontal Disease Severity: A Cross-Sectional Study. J of BMC Oral Health. 14-107. 2014.
Lindhe, J; Karring, T and Niklans, P.L. Clinical Periodontology and Implant Dentistry. 5thEdition. Blackwell Munksgaard, a Blackwell Puplishing Company. Oxford, 2008.
Carranza, F.A. Clinical Periodontology. 10th Edition. WB Sounder Company; Philadelphia, 2009.
Newman, GM., Takei, HN., Carranza, FA., and EDS. Carranza Clinical Periodontology. Philidalphia. 12th ed. 2015, pp. 96-112.
Alonso de la peńa, V., Diz Dios, P., Lojo Rocamonda, S., Tojo Sierra, R., and Rodriguez- Segade. A standardised protocol for the quantification of lactate dehydrogenase activity in saliva. S. Arch Oral Biol. 49:23-27. 2004.
Janet Moradi Haghgoo, Sara Soheilifar, Mohsen Bidgoli, Neda Rastegarfard, Maral Saremi, and Samare Kafilzade. Comparison of Whole Salivary Lactate Dehydrogenase Level in Patients With and Without Periodontal Disease. Avicenna J Dent Res. 8(4): e29026. 2016.
Manoj Upadhyay, Puneet Bhardwaj, Modi Sonamben, Nishant Agarwal, Shudhanshu Bhardwaj, and Kasper Hundal. Salivary Enzymes Potential Markers for Periodontal Disease. Int J Med Res Prof. 5(5): 134-136. 2019.
ousefimanesh, H., Maryam, R., Mahmoud, J., Mehri, GB., and Mohsen, T. Evaluation of salivary tumour necrosis factor-alpha in patients with the chronic periodontitis: A case-control study. J Indian Soc Periodontol. 17:737-740. 2013.
Heeja, S., Varghese, Hima Thomas, N. D. Jayakumar, Sankari, M., and Reema Lakshmanan. Estimation of salivary tumour necrosis factor-alpha in chronic and aggressive periodontitis patients. Contemp Clin Dent. 6 (Suppl): S152-S156. 2015.
Kurtis, B., Tuter, G., Cem, U., Akdemir, P., Erhan, F., and Belgin, B. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumour necrosis factor alpha in patients with chronic and aggressive periodontitis. J Periodontal. 76: 1849-1855. 2005.
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