The First Report on the Molecular Identification of Fresh Water Microalgae from Waterfalls of Paracelis, Mountain Province, Philippines
Keywords:16s rRNA, Chlorella pyrenoidosa, microalgae, Oscillatoriales cyanobacterium, phylogeny, rbcL
Microalgae belong to diverse groups of complex unicellular photosynthetic microorganisms typically found in the freshwater and marine environment. Towards the isolation and an attempt to report the first molecular identification of freshwater microalgae from the waterfalls of Paracelis, Mountain Province, Philippines, two microalgae were successfully isolated independently, purified, and cultivated for DNA extraction, polymerase chain reaction, and sequencing using gene 16s rRNA and rbcL markers. Based on the 16s rRNA sequence, one of the isolates has a 92.05% sequence similarity to Oscillatoriales cyanobacterium (KJ611413). On the other hand, one isolate has 98.27% sequence similarity with Chlorella pyrenoidosa (AB240145) using the rbcL. The morphological features of two microalgae were described and the phylogenetic trees were constructed using the gene marker.
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