Natural Killer Cells Phenotype in Antiretroviral Naïve HIV-1 Infected People Living in Cameroon

Authors

  • Carole Stephanie Sake Department of Microbiology, Faculty of science, University of Yaounde I, yaounde P.O. Box 812, Cameroon
  • Jules Colince Tchadji Department of animal biology and physiology, University of Yaounde I /Laboratory of vaccinology/Biobanking, the Chantal Biya International Reference Centre, Yaounde P.O. Box 3077, Cameroon
  • Georgia Ambada Department of animal biology and physiology, University of Yaounde I /Laboratory of vaccinology/Biobanking, the Chantal Biya International Reference Centre, Yaounde P.O. Box 3077, Cameroon, Department of animal biology and physiology, University of Yaounde I, yaounde P.O. Box 812/ Laboratory of vaccinology/Biobanking, the Chantal Biya International Reference Centre, Yaounde P.O. Box 3077, Cameroon
  • Loveline Ngu Department of Biochemistry, University of Yaounde I, yaounde P.O. Box 812, Cameroon.
  • Abel Lissom Department of biochemistry, Faculty of science, University of Bamenda, Bambili P.O. Box 39, Cameroon
  • Thibau Flaurant Tchouangueu Department of Microbiology, Immunology and Hematology, Faculty of Medicine and Pharmaceutical Sciences , University of Dschang, Dschang P.O. Box: 96 Cameroon
  • Larissa Djukouo Department of Biochemistry, University of Yaounde I, yaounde P.O. Box 812, Cameroon
  • François-Xavier Etoa Department of Microbiology, Faculty of science, University of Yaounde I, yaounde P.O. Box 812, Cameroon
  • Godwin Nchinda Laboratory of vaccinology/Biobanking, the Chantal Biya International Reference Centre, Yaounde P.O. Box 3077, Cameroon

Keywords:

Natural killer cells, subsets profile, ARV naïve HIV-1 infection

Abstract

The impact of antiretroviral naïve HIV-1 infection on the modulation of Natural Killer (NK) cells phenotype has not been fully assessed. This study aimed to define the phenotype of NK cell in the context of antiretroviral naïve HIV-1 infection. A total of 85 ARV naïve HIV-1 infected and 55 healthy individuals were included in the study. Purified NK cells alongside bulk Peripheral Blood Mononuclear Cells (PBMC) were surface stained with fluorochrome conjugated antibodies and samples were acquired using a BD FACS canto II flow cytometer. A down-regulation of CD56 + /CD16 - and CD56 + /CD16 + NK cells (p= 0.003), and a significant expansion (p= 0.03) of CD56 - /CD16 + NK cells subset was observed in ARV naïve HIV-1 infection. The high expression of both CD38 (p= 0.02) and HLA-DR (p=0.001) in the CD56-/CD16+ NK cells subset, shows the activation status of NK cells from HIV-1 infected people. A reduced expression of activating markers NKp44 and NKp30 and the down regulation of NKG2A was observed  in CD56+/CD16- and CD56+/CD16+ NK cells from HIV-1 infected people (p= 0.006, p= 0.009, respectively).  Antiretroviral naive HIV-1 infected people living in Cameroon show a differential modulation of NK cell phenotype relative to HIV negative controls.

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Published

2021-08-21

How to Cite

Sake , C. S. ., Tchadji, J. C. ., Ambada, G., Ngu, L. ., Lissom, A., Tchouangueu, T. F. ., Djukouo , L. ., Etoa, F.-X. ., & Nchinda, G. (2021). Natural Killer Cells Phenotype in Antiretroviral Naïve HIV-1 Infected People Living in Cameroon. International Journal of Sciences: Basic and Applied Research (IJSBAR), 60(1), 83–103. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/12993

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