Effects of the Sargassum Sp Enrichment Time on Post-Larvae White Shrimp (Litopenaeus vannamei)
Keywords:
Bio-encapsulation, Crude Extract, Hematology, Enrichment Time, White-Leg ShrimpAbstract
Sargassum sp has been applied in aquaculture and has shown promising results; however, the application in shrimp larvae through artemia bio-encapsulation and the length of Artemia enrichment is still unknown. This study aimed to evaluate the effect of Sargassum sp and its duration of enrichment on Artemia sp on the survival, growth, and immunity of white-leg shrimp (Litopenaeus vannamei) post-larvae. This study was an experimental study using a completely randomized design (CRD). The treatment was the duration of Artemia enrichment in Sargassum sp crude extract fed to vaname shrimp post larvae, namely: an enrichment time of 30 minutes (A), an enrichment time of 60 minutes (B), an enrichment time of 90 minutes (C), and without Sargassum sp extract (control) (D). The test animals were 1,440 PL1 stage white-leg shrimp (L. vannamei), stocked into 12 jars, each containing 2 L of water with a density of 60 shrimp/L (120 shrimp/jar). Daily feeding was carried out twice with Artemia sp and 4 times with flour feed, reared for 20 days. An environmental stress test was then carried out at a pH of 5 on the 20th day and the shrimp were observed 24 hours after the stress test. Observation parameters consisted of larvae survival, growth, and immunity. The results showed that the bio-enrichment of Artemia sp with Sargassum sp extract could improve the growth performance, survival, and hematology of white-leg shrimp after being reared for 20 days. Similarly, the environmental stress testing at a pH of 5 showed that the shrimp larvae had high survival rates and hematology, especially in the treatment with an enrichment duration of 90 minutes. Therefore, it was concluded that the bio-enrichment of Artemia sp with Sargassum sp extract could improve the survival rate, growth, and immunity profile of white-leg shrimp post larvae.
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