Neuroprotective Role of Glutathione against Hydrogen Peroxide Induced Toxicity to the Neuronal Cells in Culture
Keywords:
Oxidative stress, Hydrogen peroxide, Glutathione(GSH), SH-SY5Y, Comet assay, DNA fragmentation assay.Abstract
Neuropathophysiology research is receiving considerable attention. Studies have demonstrated that under oxidative stress, reactive oxygen species (ROS) generated at high levels inducing cellular and DNA damage, thereby resulting in apoptosis of neuronal cells. This is implicated in the etiology of several neurodegenerative and neurodevelopmental disorders. This study was undertaken to examine the role of glutathione as a Neuroprotective bioactive compound on hydrogen peroxide-induced apoptosis. Assessment of DNA damage with the help of Comet assay (single cell gel electrophoresis) and DNA fragmentation Assay were carried out on cultured SH-SY5Y neuroblastoma cells. The treatment with glutathione markedly attenuated hydrogen peroxide-induced cell viability loss and apoptotic neuronal cell death. These results provide evidence that glutathione may act as a significantly bioactive compound and support the possibility that it may be important in health and disease, and for protection against DNA damage by oxidative stress.
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