Extraction and Identification of Indole-3-Acetic Acid Synthesized by Rhizospheric Microorganism

Abstract

IAA is a key regulator of plant growth and development. The growth regulation is mainly dependent of the change of free Indole 3 acetic acid levels in the target tissues, quantification of indole-3-acetic acid (IAA), the most abundant natural auxin, is indispensable in the study of auxin action. Currently; spectrophotometry techniques like HPLC are technically the best methods to measure Indole 3 acetic acid, due of high sensitivity and specificity. However, its high cost for setting and maintenance makes it difficult for daily use in ordinary laboratory. Therefore, establishment of a standard method to quantify IAA based on different spectrophotometric techniques, ensure the specificity and concentration of auxins like indole 3 acitic acid.