Prevalence of Entamoeba histolytica and Giardia lamblia in District Hangu of Khyber Pakhtunkhwa, Pakistan

Authors

  • Farzana Raza
  • Sultan Ayaz
  • Ambreen Akram
  • Taqdir Ali
  • Sidra Saeed
  • Laila Rubab
  • Noor ul Akbar

Keywords:

Giardia lamblia, E.histolytica, HSP, PCR

Abstract

Giardia lamblia and Entamoeba histolytica are two important parasites of the gastrointestinal tract causing gastroenteritis in human population. The present study is carried out to check the prevalence of these two parasites in the stream and open well water of district Hangu of Khyber Pakhtunkhwa.200/two hundred sample were examined through PCR which showed 26.5% (53/200) and 22.5%(45\200) positive results for Giardia lamblia and E.histolytica respectively. The PCR product for Giardia lamblia was 163bp HSP (heat shock protein) and for Entamoeba histolytica was135bp small fragment of DNA.

References

Bouzid, M., Steverding, D. and Tyler, K.M. (2008) Detection and surveillance of waterborne protozoan parasites COBIOT-534; 19:1

Virkutyte, J. Sillanpa, M.(2006). Chemical evaluation of potable water in Eastern Qinghai Province, China: Human health aspects. University of Kuopio, Laboratory of Applied Environmental Chemistry, P.O. Box 181, FIN-50101 Mikkeli, Finland. Environ. Int., 32: 80-86.

World Health Organization, 2004. Guidelines for Drinking-water quality. 3rd edition, Geneva. pp: 121-144.

Brandborg, L. L., R. Owen, and R. Fogel. 1980. Giardiasis and traveler's diarrhea. Gastroenterology 7b 8:1602-1614.

Yolken, R. H., Stopa, P. J., Harris, C. C., (1980). Enzyme immunoassay for the detection of rotavirus antigen and antibody, p. 692-699. In N. R. Rose and H. Friedman (ed.), Manual of clinical immunology, 2nd ed. American Society for Microbiology, Washington, D.C.

Eligio Garc

Ponce-Macotela, M., G.E. Peralta-Abarca & Mart

Kirner, J.C., Littler, J.D., Angelo, L.A. 1978 A waterborne outbreak of giardiasis in Camas, Washington. Journal of American Water Works Association; 70:35-40.

Katz DE, Heisey-Grove, D., Beach M, Dicker, R.C., Matyas, B.T.(2006).,. Prolonged outbreak of giardiasis with two modes of transmission. Epidemiol Infect 134:935-941.

Wolfe, M.S. 1992. Giardiasis. Clinical Microbiology Reviews, 5(1):93-100

Benenson AS (ed) . (2011),: Control of Communicable diseases in Man.Washington DC:

WHO, 1997. WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico 28-29 January, (1997). Epidemiol Bull 18, 13-14.

Upcroft, P., Upcroft, J.A., (2001). Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin Microbiol Rev 14(1):150-164.

Pennardt ,A. Giardiasis.[http://www.emedicine.com/EMERG/topic215.htm].

Ortega, Y.R., Adam, R. (1997). Giardia: overview and update. Clin Infect Dis 25:545-549.

Tanyuksel, M., H. Tachibana, and W.A. Petri, Jr. 2001. Amebiasis,an Emerging Disease, p. 197-212. In W.M. Scheld, W.A. Craig,and J.M. Hughes (eds.), Emerging Infections . American Society for Microbiology, Washington D.C.nd Microsporidia. Parasitology 117 (Suppl),S143-S159.

Tanyuksel, M. & Petri, W.A. (2003).Laboratory diagnosis of amebiasis. Clinical Microbiology Reviews 16: 713-729.

Agha rodina, Al. & Teoderesco, I. (2002) prevalence of intestinal parasites in three localities of Ghaza governorates-palestine.Archi.Pubi.Hith.;60 :363-370.

Fotidar, R.,Stark, D.,Beebe, N.,et al. 2007. Laboratory diagnostic techniques for entamoeba species Clin Microbial Rev.jul;20(3): -51132.

Ratanapo, S., Mungthin, M., Soontrapa, S., Faithed, C., Siripattanapipong, S.,Rangsin, R., Naaglor, T., Piyaraj, P., Taamasri, P., Leelayoova, S., (2008). Multiple modes of transmission of giardiasis in primary schoolchildren of a rural community, Thailand. Am J Clin Nutr 78(4):611-615.

Stanley, S.L., Jr., 2003. Amoebiasis. Lancet 361, 1025-1034.

Clark, C.G., 2000. Cryptic genetic variation in parasitic protozoa. J Med Microbiol 49:489-491.

Gonin, P., Trudel, L. 2003. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay. J Clin Microbiol 41: 237-241.

Kasprazak, W. and Pawlowski. (1989). Zoonotic Aspect of Giardiasis. A review, Veterinary Parasitology 32:101 108.

Guerrant, R.l. 1970.Cryptosporidiosis: an emerging, highly infectious threat. Emerging Infectious Diseases, 3: 51-57.

Walsh, J.A. (1986). Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Reviews of Infectious Diseases, 8:228

Walsh, J.A., (1986). Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Reviews of Infectious Diseases, 8:228

Ayaz, S. , Khan, S., Niaz, S., Bibi, F., Shamas, S. and Akhtar, . (2011) M. Prevalence of Zoonotic Parasites in Drinking Water of Three Districts of Khyber Pakhtunkhwa Province, Pakistan Pak. J. life soc. Sci9(1): 67-69

Hernandez, C.F. and Avendendo, L. 2001. Simple modification of the Baermann method for diagnosis of Strongyloidiasis. Mem Inst Oswaldo Cruz, Rio de Janeiro, 96: 805-807.

Kaur, R., Rawat, D., Kakkar, M., Uppal, B., and Sharma, V.K. (2002). Intestinal parasites in children with diarrhea in Delhi, India. Southeast Asian. J. Trop. Med. Public Health. 33: 725-729.

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Published

2015-04-09

How to Cite

Raza, F., Ayaz, S., Akram, A., Ali, T., Saeed, S., Rubab, L., & ul Akbar, N. (2015). Prevalence of Entamoeba histolytica and Giardia lamblia in District Hangu of Khyber Pakhtunkhwa, Pakistan. International Journal of Sciences: Basic and Applied Research (IJSBAR), 21(2), 147–159. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/3594

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