Regulatory T Cells CD4+ CD25+ Expression in Biliary Atresia Mice Model after Exposure with Rhesus Rotavirus (RRV)
Keywords:
mice model biliary atresia, rhesus rotavirus (RRV), regulatory T cells, CD4 CD25 .Abstract
Billiary atresia (BA) is still a major challenge for clinicians, as without surgical intervention, more than 80% of patients will develop liver cirrhosis, or die within 2 years of life. The pathogenesis is still unknown, but a theory of primary perinatal hepatobiliary viral infection followed by auto- immune-mediated bile duct injury had been hypothesized. The objective of this study is to determine the change of regulatory T cells and inflammatory reaction in liver after rhesus rotavirus (RRV) infection in BA mice model. Two groups of newborn mice, those were RRV group consisting of 24 newborn mice injected by RRV 1.5 x 106 PFU intraperitoneally less than 24 hours after birth, and control group comprising of 24 newborn mice injected with buffered saline were used as BA model. The expression of Tregs and infiltration of inflammatory cells in the liver of the two groups of newborn mice were studied on day 3, 7, 14, and 21 after birth.
The results shows that tregs expressions of RRV group on day 3, 7, 14 and 21 were significantly higher than control group (p<0.05). There were also significant increases of Tregs expressions on day by day, both in RRV group (p<0.001), as well as in control groups (p=0.002). The excessive infiltration of inflammatory cells and narrowing of bile duct lumens were detected on day 14. As a conclusion we can say that the Induction of Rotavirus (RRV) in newborn mice model BA increases the expressions of Tregs, and may cause damage of bile ducts within two weeks of life.
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