Encapsulation of Bacteria-Derived Auxin, Cytokinin and Gibberellin and its Application in the Micropropagation of Coconut (Cocos nucifera L. var Makapuno)

Authors

  • Ronald Arlet P. Villaber nstitute of Chemistry, University of the Philippines Los, Ba
  • Florinia E. Merca nstitute of Chemistry, University of the Philippines Los, Ba
  • Lilia M. Fernando National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los, Ba
  • Teofila D.C. Villar nstitute of Chemistry, University of the Philippines Los, Ba
  • Constancio C. de Guzman Crop Science Cluster, University of the Philippines Los, Ba

Keywords:

Encapsulation, tissue culture, coconut, plant growth regulator.

Abstract

Improved solubility of the PGR-loaded liposomes in aqueous solution was also observed. The PGR-loaded liposomes showed controlled-released behavior of the active component at pH 5.0 and 7.0. Higher stability of the encapsulated PGRs was observed at 0 - 4

References

. Woodward, a. W., and b. Bartel, 2005. Auxin: regulation, action, and interaction. Annals of botany vol. 95: pp 707-735.

. Difuntorum-tambalo, d., e. S. Paterno, w. Barraquio, and i. M. Duka, 2006. Identification of an indole-3-acetic acid-promoting plant growth-promoting bacterium (pgpb) isolated from the roots of centrosema pubescens benth. The philippine agricultural scientist 89(2): 149

. Ereful, n. C., e. S. Paterno and f. E. Merca, 2007. Assessment of cytokinin production in some plant growth-promoting bacteria. Asia life sciences 16(2): 137 - 152

. Fernando, l. M., f. E. Merca and e. S. Paterno, 2010. Isolation and partial structure elucidation of gibberellin produced by plant growth promoting bacteria (pgpb) and its effect on the growth of hybrid rice (oryza sativa l.). Philippine journal of crop science 35(2): 12

. Wilkinson, m. G. And k. N. Kilcawley, 2005. Mechanism of incorporation and release of enzymes into cheese during ripening. Int dairy j 15:817

. Reis, c. R., r. J. Neufeld, a. J. Ribeiro and f. Veiga, 2006. Nanoencapsulation i. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomed journal 2 (1): 8-21

. Villareal, r. L., 2014. The coconut scale insect (csi) outbreak in the philippines. Star science, the philippine star

. Pardee, a. B., f. Jacob and j. Monod, 1959. The genetic control and cytoplasmic expression of

. King, e. O., m. K. Ward, and d. E. Raney, 1954. Two simple media for the demonstration of phycocyanin and fluorescin. J lab clin med 44, 301

. Wu, y. And y. Wang, 2004. Fractionation of crude soybean lecithin with aqueous ethanol. J amer oil chem soc. 81:7

. Jangle, r. D., v.p. magar and b. N. Thorat, 2013. Phosphatidylcholine and its purification from raw de-oiled soya lecithin. Separation and purification technology 102: 187-195

. De, a. And d. N. Venkatesh, 2012. Design and evaluation of liposomal delivery system for l-asparaginase. Journal of applied pharmaceutical science 02(08): 112-117

. Mozafari, m. R., 2006. Nanocarrier technologies: frontiers of nanotherapy. Springer, the netherlands

. Gulati, m., m. Grover, m. Singh, and s. Singh, 1998. Study of azathioprine encapsulation into liposomes. J microencapsul. 15:485

. Panwar, p., p. Bhumika, p. C. Lakhera and k. P. Singh, 2010. Preparation characterization, and in vitro release study of albendazole-encapsulated nanosize liposomes. Int j nanomedicine 5: 101

. Perez-nu

. Tahara, k., s. Fujimoto, f. Fujii, y. Tozuka, t. Jin and h. Takeuchi, 2013. Quantum dot-loaded liposomes to evaluate the behavior of drug carriers after oral administration. Journal of pharmaceutics 10.1155/2013/848275

. Dubertret, b., p. Skourides, d. J. Norris, v. Noireaux, a. H. Brivanlou, and a. Libchaber, 2002. In vivo imaging of quantum dots encapsulated in phospholipids micelles. Science 298:1759

. Azpeitia, a., j. L. Chan, l. Sa

. Shih-yung, h. 2010. Iaa production by streptomyces scabies and its role in plant microbe interaction. Msc thesis, cornell university.

. Arshad, m., and w. T. Frankenberger, jr., 1992. Microbial production of plant growth regulators. In: metting fb jr(eds). Soil microbial ecol, marcel dekker inc., new york. Pp: 307-347.

. Ahmad, f., i. Ahmad and m. S. Khan, 2006. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Micres 163(2): 173-181

. Zhender, g. W., c. Yao, j. F. Murphy, e. R. Sikora, j. W. Kloepper, d. J. Schuster and j. E. Polston, 1990. Microbe-induced resistance against pathogens and herbivores: evidence of effectiveness in agriculture

. Melzak, k. A., s. A. Melzak, e, gizeli and j. L. Toca-herrera, 2012. Cholesterol organization in phosphatidylcholine liposomes: a surface plasmon resonance study. Materials 5(11): 2306

. Machado, a. R., l. M. De assis, m. I. R. Machado and l. A. De souza-soares, 2014. Importance of lecithin for encapsulation processes. African j of food science 8(4): 176-183

. Mozafari, m. R. And s. M. Mortazavi, 2005. Liposomes: from fundamentals to recent developments. Oxford, uk: trafford pub. Ltd.

. Mozafari, m. R., and v. Hasirci, 1998. Mechanism of calcium ion induced multilamellar vesicle-dna interaction. J microencapsul 15:55

. Colletier, j.-p., b. Chaize, m. Winterhalter and d. Fournier, 2002. Protein encapsulation in liposomes: efficiency depends on interactions between protein and phospholipid bilayer. Bmc biotechnology 2: 9

. Cadena, p. G., m. A. Pereira, r. B. S. Cordeiro, i. M. F. Cavalcanti, b. B. Neto, m. Dc. C. B. Pimentel, j. L. L. Filho, v. L. Silva and n. S. Santos-magalhaes, 2013. Nanoencapsulation of quercetin and resveratrol into elastic liposomes. Biochemica et biophysica acta 1828: 309-316.

. Chaudhry, q., m. Scotter, j. Blackburn, b. Ross, a. Boxall and l. Castle, 2008. Applications and implications of nanotechnologies for the food sector. Food add cont 25(3):241

. Osanai, s. And k. Nakamura, 2000. Effects of complexation between liposomes and poly(malic acid) on aggregation and leakage behavior. Biomaterials 21(9): 867-876.

. Drummond, d. C., m. Zignani and j. Leroux, 2000. Current status of ph-sensitive liposomes in drug delivery. Prog lipid res 39(5): 409-460.

. Ho, r. J. Y., b. T. Rouse, and l. Huang, 1987. Target-sensitive immunoliposomes as an efficient drug carrier for antiviral activity. The journal of biological chemistry 262(29): 13973-13978.

. Henriksen, i., s. A. Sande, g. Smistad, t. Agren and j. Karlsen, 1995. In vitro evaluation of drug release kinetics from liposomes by fractional dialysis, int. J. Pharm. 119: 231

. Paveli, z., n. Basnet and r. Schubert, 2001. Liposomal gels for vaginal drug delivery, int. J. Pharm. 219: 139

. Batista, c. M., c. M. B. Carvalho, and n. S. S. Magalh

. Martinelli, l. E. Candioli, d. Costa, v. Poletti, and n. Rascio, 2001. Morphogenic competence of vitis rupestris s. Secondary somatic embryos with a long culture history. Plant cell rep. 20:279

. Puigderrajols, p., g. Mir, and m. Molinas, 2001. Ultrastructure of early secondary embryogenesis by multicellular and unicellular pathways in cork oak (quercus suber l.). Ann. Bot. 87:179

. Bhagya, n. And k. R. Chandrashekar, 2013. Effect of growth regulators on callus induction from cyclea peltata (lam.) Hook. F. Thoms. Asian j pharm clin res.

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Published

2016-05-10

How to Cite

P. Villaber, R. A., E. Merca, F., M. Fernando, L., D.C. Villar, T., & C. de Guzman, C. (2016). Encapsulation of Bacteria-Derived Auxin, Cytokinin and Gibberellin and its Application in the Micropropagation of Coconut (Cocos nucifera L. var Makapuno). International Journal of Sciences: Basic and Applied Research (IJSBAR), 27(1), 37–56. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/5276

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