Inhibitory Concentration and Minimum Contact Time Gambir Extract (Uncaria gambier Roxb) Against Bacterial Growth Enterococcus faecalis

Authors

  • Hafsah Katu Departemen of Conservation, Dentistry Faculty, Hasanuddin University
  • Sumintarti Sumintarti Department of Mouth Disease Science, Dentistry Faculty, Hasanuddin University
  • Indya Kirana Mattulada Departemen of Conservation, Dentistry Faculty, Hasanuddin University
  • Rasmidar Samad Department of Community Dentistry, Hasanuddin University
  • Moch Hatta Department of Microbiology, Medical Faculty, Hasanuddin University
  • Suryani As Nutritional Department, Medical Faculty, Hasanuddin University

Keywords:

Minimal Inhibitory Concentration, Time minimal contact, Extract Gambir, E. Faecalis.

Abstract

Antibacterial activity is determined based on the time zone diameter hambat.Analisis contact and SPSS version 16.0 for windows with One-way ANOVA test and LSD on (p <0.05). Results indicated that Statistical analysis showed a 1% concentration and contact time of 24 hours, effectively inhibits the growth of bacteria E.faecalis. in conclusion, Gambir extract effectively inhibits the growth of bacteria E. faecalis.

Enterococcus faecalis (E.Faecalis) gram-positive bacteria commonly found in endodontic retreatment cases. Extract Gambir, a type of dried sap from the leaves and young stems of plants gambier (Uncaria gambier Roxb) contains catechins, which are potent antibacterial and anti fungal with minimal side effects. This study aimed to determine the inhibitory concentrations and minimal contact time Gambir extract on the growth of the bacteria E. faecalis. Research applied a design with lab experiments, conducted on May 30-June 16, 2011 at the Laboratory of Pharmacognosy-Phytochemicals Faculty of Pharmacy and Laboratory of Microbiology, Faculty of Medicine, University of Hasanuddin. Materials and methods; 600 gr Gambir which has been crushed, extracted with reflux and rotary method. Minimal Inhibitory Concentration is determined with 5 ml of 5.25% NaOCl as a positive control and sterile distilled water negative control.

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Published

2016-06-14

How to Cite

Katu, H., Sumintarti, S., Mattulada, I. K., Samad, R., Hatta, M., & As, S. (2016). Inhibitory Concentration and Minimum Contact Time Gambir Extract (Uncaria gambier Roxb) Against Bacterial Growth Enterococcus faecalis. International Journal of Sciences: Basic and Applied Research (IJSBAR), 27(3), 239–246. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/5912

Issue

Vol. 27 No. 3 (2016)

Section

Articles

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