Preventive and Curative Effects of Phyllanthus niruri Allium sativum Combination on Tiger Grouper Epinephelus fuscoguttatus Infected by Vibrio alginolyticus
Keywords:
Vibrio alginolyticus, Phyllanthus niruri, Allium sativum, tiger grouper.Abstract
The aim of this study was to evaluate the preventive and curative effects of supplementation of the combination of stone-breaker (Phyllanthus niruri) and garlic (Allium sativum) through the feed to control bacterial diseases on tiger grouper juvenile (Epinephelus fuscoguttatus) caused by Vibrio alginolyticus. The extract was prepared in five combination doses with two replications: 15+20, 15+25, 20+20, 20+25 and 25+20 g L-1. These combination doses were tested at in vitro test to observe the antibacterial activity of medicinal plants against V. alginolyticus (104 CFU mL-1, 0.1 mL). The in vivo test was conducted using CRD (completely randomized design) consisting of four treatments and three replications; negative control, positive control, preventive, and curative treatment. Tiger grouper juveniles (length 7.74
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