Dengue Virus Type 3 (DENV-3) Distribution in Tissues of Pig-tailed Macaque (Macaca nemestrina) Post Infection Using Immunohistochemistry Technique
Keywords:
DENV-3, Macaca nemestrina, immunohistochemistry.Abstract
Nonhuman primates play as an indispensable animal model in biomedical research for studying a variety of human health issues, diseases and disorders, therapies, and preventive strategies. In this study, we used pig-tailed macaques (Macaca nemestrina) as an experimental animal to study dengue type-3 virus (DENV-3) infection. We evaluated DENV-3 distribution and replication sites after a primary infection in all collected tissue by immunohistochemistry to localize viral protein. Significant gross lesions were not seen in any of the examined pigtailed macaques. However, microscopic lesions were present in variable degrees of severity in multiple tissues: liver, stomach, spleen and lymph nodes as evaluated by histopathological analysis. Viral protein was demonstrated in reactive lymphoid cells in spleen, thymus, axillary lymph node, inguinal lymph node, mesenteric lymph node and submandibular lymph node. In general, evidence for the presence of viral protein in various tissues after DENV-3 infection reveals that M. nemestrina is susceptible to the infection and could serve as a good alternate model to evaluate the replication of dengue virus in tissues.
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