Expression Analysis of mRNA Interleukin-8 and (mRNA) Monocytes Chemo Attracting Protein-1 (mRNA MCP-1) in Bruise Age Determination
Keywords:
mRNA IL-8, mRNA MCP-1, bruises.Abstract
The number of incidents of injuries in the hospital for a criminal case requires examination report including wound age in accordance with Article 133 Criminal Procedure Code. This study aims to determine the bruise age of the wound through mRNA expression of IL-8 and MCP-1 mRNA in body bruises. Using qPCR techniques: extraction of mRNA of IL-8 and MCP-1 mRNA from blood samples by the method of Boom. Results showed differences in mRNA expression of IL-8 and MCP-1 in healthy bodies (ie 8.70 and 12.74 Log Log). There is an increased expression of mRNA of IL-8 and MCP-1 gradually by the age of the wound 14 hours, 28 hours and 34 hours for mRNA expression of IL-8, while 28 hours and 34 hours for mRNA expression of MCP-1. There are variations in mRNA expression of IL-8 and MCP-1 mRNA in each age group wound. This suggests that there are several other factors that affect mRNA expression of IL-8 and MCP-1 is not yet known, so further research is still needed.
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