The Effect of the Extracts of Betel (Piper betel) Leaves on the Content of IL-1? as the Inflammatory Cytokine in Experimental Mice Using an Acute Wound Healing Modeling
Keywords:
Piper betel, interleukin-1, wound healing.Abstract
This study aims to determine the effect of ethyl acetate derived from the leaves extraction of betel (Piper betel) on the content of Interleukin-1? (IL-1?) in experimental mice using an acute wound healing modeling. The experimental design used randomized post test only group with 72 experimental mice which divided into 4 groups and each group consisted of 6 mice with three replications. The negative control group (I) was given vaseline after incision. The positive control group (II) was given bioplasenton. The third group (III) was topically given the extracts of betel leaves at 2% concentration and the fourth group (IV) was given the extracts of betel leaves at 3% concentration after incision respectively. The determination of IL-1? applied Immunoassay Quantikine Elisa (IQE).
The quantitative analysis (statistical test) used complete randomized design using one-way anova with Spearman
References
MacLellan, L., Gardner, G., & Gardner, A. (2 002). Designing the future in wound care: the role of the nurse practitioner. Primary Intention, Vol.10 No.3 , 97-112.
Alam, B., Akter, F., Parvin, N., Pia, R. S., Akter, S., Chowdhury, J., et al. (2013). Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J Phytomed, Vol. 3, No. 2, Spring 2013 , 112
Bhide SV, Zariwala MBA, Amonlar AJ, Azuine MA. Chemo-preventive efficacy of betel leaf extract against benzo(?)pyrene induced fore-stomach tumors in mice. J Ethnopharmacol. 1991;34:207
Garg SC, Jain R. Biological activity of the essential oil of Piper betel L. J Essen Oil Res. 1992;4:601
Singh M, Shakya S, Soni VK, Dangi A, Kumar N, Bhattacharya SM. The n-hexane and chloroform fractions of Piper betel L. trigger different arms of immune responses in BALB/c mice and exhibit antifilarial activity against human lymphatic filarid Brugia malayi. Int Immunopharmacol. 2009;9:716
Choudhary D, Kale RK. Antioxidant and non toxic properties of Piper betel leaf extract: in vitro and in vivo studies. Phytother Res. 2002;16:461
Santhakumari P, Prakasam A, Pugalendi KV. Modulation of oxidative stress parameters by treatment with Piper betel leaf in streptozotocin induced diabetic rats.Indian J Pharmacol. 2003;35:373
Shun CY, Chau JW, Jing JL, Pei LP, Jui LH. Fen PC. Protection effect of Piper betle leaf extract against carbon tetrachlorideinduced liver fibrosis in rats. Archives of Toxicology. 2007; 81:45?55.
Lei D. Antioxidant and antiplatelet effect of aqueous inflorescence Piper betel extract. Journal of Agricultural Food and Chemistry. 2003; 51: 2083?8.
Majumdar B, Chaudhuri SR, Roy A. Potent antiulcerogenic activity of ethanol extract of leaf of Piper betel Linn. by antioxidative mechanism. Indian Journal of Clinical Biological Chemistry. 2002; 17: 49?57.
Adhikari P, Chowdhury D, Baneerji J, Chatterjee A. Antifertility effect of crude alcoholic extract of Piper betel stalk. Indian Journal of Physiology and Allied Sciences. 1998; 52: 22?7.
Adhikary P, Banerji J, Choundhuri D, Das AK, Deb CC, Mukherjee P, Chatterjee SR. Effect of oral administration of stalk of leaves of Piper betel Linn on oestrous cycle and its antifertility activity in rats. Indian Journal of Physiology and Allied Sciences. 1990; 44:116?23.
Chen SJ, Wo BN, Yeh JL, Lo YC., Chen IS, Chen IJ. C?fiber evoked autonomic cardiovascular effect of after injection of Piper betel inflorescence extracts. Journal of Ethnopharmacology. 1995;43: 183?8.
Amonkar AJ, Padma PR, Bhide S. Protective effect of hydroxychavicol, a phenolic component of Piper betel leaf against the tobacco?specific carcinogens. Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis. 1989;210: 249?53.
Varier PS. Piper betel Linn. In: Varier PS. (ed) Indian medicinal plants. Kottakkal : Oreint Longman. 1997; 8: 279?283.
Agarwal T, Singh R, Shukla AD, Waris I, Gujrati A. Comparative analysis of antibacterial activity of four Piper betel varieties. Adv Appl Sc Res. 2012;3:698
Majumdar B, Chaudhuri SGR, Ray A, Bandyopadhyay SK. Effect of ethanol extract of Piper betel Linn leaf on healing of NSAID-induced experimental ulcer a novel role of free radical scavenging action. Indian J Exp Biol. 2003;41:311
Saravanan R, Prakasam A, Ramesh B, Pugalendi KV. Influence of Piper betel on hepatic marker enzymes and tissue antioxidant status in ethanol-treated wistar rats. J Med Food. 2002;5:197
Ratnasooriya WD, Jayawardena KGI, Premakumara GAS. Antimotility effects of Piper betel (L) leaf extract on washed human spermatozoa. J Nat Sci Council.1990;18:53
Freedberg IM, Tomic-Canic M, Komine M, Blumenberg M. Keratins and the keratinocyte activation cycle. J Invest Dermatol 2001; 116: 633
Kupper TS, Deitch EA, Baker CC, Wong WC. The human burn wound as a primary source of interleukin-1 activity. Surgery 1986; 100: 409
Murphy GM, Dowd PM, Hudspith BN, Brostoff J, Greaves MW. Local increase in interleukin-1-like activity following UVB irradiation of human skin in vivo. Photodermatol 1989; 6: 268
Bochner BS, Charlesworth EN, Lichtenstein LM, Derse CP, Gillis S, Dinarello CA, Schleimer RP. Interleukin-1 is released at sites of human cutaneous allergic reactions. J Allergy Clin Immunol 1990; 86 (6 Pt 1): 830
Mizutani H, Schechter N, Lazarus G, Black RA, Kupper TS. Rapid and specific conversion of precursor interleukin 1 beta (IL-1 beta) to an active IL-1 species by human mast cell chymase. J Exp Med 1991; 174: 821
Chan LS, Hammerberg C, Kang K, Sabb P, Tavakkol A, Cooper KD. Human dermal fibroblast interleukin-1 receptor antagonist (IL-1ra) and interleukin-1 beta (IL-1 beta) mRNA and protein are co-stimulated by phorbol ester: implication for a homeostatic mechanism. J Invest Dermatol 1992; 99: 315
Wood LC, Elias PM, Calhoun C, Tsai JC, Grunfeld C, Feingold KR. Barrier disruption stimulates interleukin-1 alpha expression and release from a pre-formed pool in murine epidermis. J Invest Dermatol 1996; 106: 397
Lundqvist EN, Egelrud T. Biologically active, alternatively processed interleukin-1 beta in psoriatic scales. Eur J Immunol 1997; 27: 2165
Zepter K, Haffner A, Soohoo LF, De Luca D, Tang HP, Fisher P, Chavinson J, Elmets CA. Induction of biologically active IL-1 beta-converting enzyme and mature IL-1 beta in human keratinocytes by inflammatory and immunologic stimuli. J Immunol 1997; 159: 6203
Murphy JE, Robert C, Kupper TS. Interleukin-1 and cutaneous inflammation: a crucial link between innate and acquired immunity. J Invest Dermatol 2000; 114: 602
Corsini E, Primavera A, Marinovich M, Galli CL. Selective induction of cell-associated interleukin-1alpha in murine keratinocytes by chemical allergens. Toxicology 1998; 129: 193
Giraldo S, Jesus Shancez, Quentin Felty, Deodutta Roy. 2008. Atlas of Genytogenetics in Oncology and Haematology.
Spielmann, H.E., Genschow, M., Leibsch, M. & Halle, W., 1999,
WHO. (2000). General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine. Geneva: World Health Organization.
Simonsen, L., Petersen, M.B. & Groth, L., 1992,
Kun, Li. et al. (2011). Tannin Extract From Immature Fruits of Terminalia Chebula Fructuz Retz. Promote Cutaneous Wound Healing In Rats. BMC Complementary and Alternative Medicine , 11: 86-95.
Behm. 2011.
Patil, D. D., & Wadhawa, G. C. (2014). Antioksidant Effect of The Stem And Leaves of Piper Betle. International Journal of Pharmacy, 4(2) , 73-75.
Mercandetti, M. (2015). Wound Healing and Repair. Accessed on 27-7-2016 from http://emedicine.medscape.com: http://emedicine.medscape.com/article/1298129-overview.
Kondo & Oshima, 1996. The Dynamics of Inflammatory Cytokines in the Healing Process of Mouse Skin Wound: A Preliminary Study for Possible Wound Age Determination. Springer.
Chitra and Vidya.2006. Dose Dependent Effect of Piper Betel Linn Leaf Extract on Erythrocytes of Experimental Mice. Sri Ramachandra Journal of Medicine Vol. 1. 1 September 2006.
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