Detection of Goat Brucellosis in Several Districts Showing Bovine Brucellosis Seropositivity in Java Island, Indonesia
Keywords:
Brucella, brucellosis, goat, serology, seropositive.Abstract
Brucellosis is a zoonotic disease that infects multiple species of animals. The species of Brucella that infects goats is Brucella melitensis.
The proportion of brucellosis seropositive among goats in several districts showing bovine brucellosis seropositivity in Java Island, Indonesia was investigated in 2015. Serum samples were collected from 214 goats with judgment sampling from quarantine exits, livestock wholesalers and goat farms in Java Island. The Rose Bengal test (RBT), complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA) were used to detect antibodies against Brucella. Brucella was identified from seropositive samples using bacterial culture and polymerase chain reaction (PCR). From serological examination of the goat samples, only two samples showed positive results for RBT, and three were positive for CFT, but none of those samples showed positive results for ELISA. Brucella spp bacteria were not detected by bacterial culture and PCR from vaginal swabs or milk samples. The proportion of Brucellosis seropositive amongst tested goats was low (2.3%;95% Confidence Interval (CI); 0.0209 - 0.0251) (5/214). The present of seropositive goats indicates the previous exposure of brucellosis which genarated antibodies. However, the species of Brucella that had infected them remains unknown.
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