Molecular Analysis of rrs Gene Mutation on Detecting The Resistance of Kanamycin from the Clinical Isolates of Tuberculosis Patient
Keywords:
Molecular, rrs Gene Mutation, Resistance of Kanamycin, Tuberculosis Patient.Abstract
The resistance of kanamycin (KAN) as a second-line drug treatment for tuberculosis is associated with mutations in the 16S rRNA gene (rrsgene).rrsgene mutations occur at different locus causing changes in codons and amino acids.This study aimed to analyze mutations in the rrs gene and its relationship with the resistance of KANin isolate clinical sample ofMycobacterium tuberculosis(MTB). The isolate clinical sample of MTB was obtained from the TB HUM-RC laboratory. The analysis of rrs gene mutation was conducted using PCR sequencing.
The results described that from 93 samples isolates, 51 (54.8%) samples shown multidrug resistant (MDR) while 42 samples shown (45.2%) non-MDR. Drug sensitivity test (DST) from the second line drugs samples isolates7 of 93 samples (7.5%) that shown resistance with KAN. The result of PCR sequencing shown6 (6.4%) of 93 isolates samples had mutations rrsgenein the locus of T1521C (1.1%), G1484C (1.1%), A1401G (2.1%), G940T (1.1%), and A514C (1.1%). Based on that gene locus, an unreported new mutation in the locus of rrs gene, called G940T,was discovered.The results of statistical analysis based on the number of mutations in each of anti-tuberculosis drugs indicate the p<0.05,described a relatioship between the mutation of rrs gene with the resistance of KAN. In conclusion, significance and impact of study, there is an association between the mutation in the rrs gene and the resistance to the second-line drugs of KAN in clinical isolates of MTB.Thus, samples of clinical isolates of MTB could be used to detect any resistance of KAN in advance.
References
Engstr
Campbell, P. J., Morlock, G. P., Sikes, R. D., Dalton, T. L., Metchock, B., Starks, A. M., Hooks, D. P., Cowan, L. S., Plykaitis, B. B. and Posey, J. E. (2011). Molecular detection of mutations associated with first- and second-line drug resistance compared with conventional drug susceptibility testing of Mycobacterium tuberculosis.Antimicrobial Agents and Chemotherapy55(5), 2032
Georghiou, S. B., Magana, M., Garfein, R. S., Catanzaro, D. G., Catanzaro, A. and Rodwell, T. C. (2012). Evaluation of genetic mutations associated with Mycobacterium tuberculosis resistance to amikacin, kanamycin and capreomycin: a systematic review. PloS One7(3), e33275. 10.1371/journal.pone.0033275.
Bauskenieks, M., Pole, I., Skenders, G., Jansone, I., Broka, L., Nodieva, A., Ozere, I., Kalvisa, A., Ranka, R. and Baumanis, V. (2015). Genotypic and phenotypic characteristics of aminoglycoside-resistant Mycobacterium tuberculosis isolates in Latvia. Diagnostic Microbiology and Infectious Disease81(3), 177
Suzuki, Y., Katsukawa, C., Tamaru, A., Abe, C., Makino, M., Mizuguchi, Y. and Taniguchi, H. (1998). Detection of kanamycin-resistant Mycobacterium tuberculosis by identifying mutations in the 16S rRNA gene. Journal of Clinical Microbiology36(5), 1220
Jugheli, L., Bzekalava, N., de Rijk, P., Fissette, K., Portaels, F. and Rigouts, L. (2009). High level of cross-resistance between kanamycin, amikacin, and capreomycin among Mycobacterium tuberculosis isolates from Georgia and a close relation with mutations in the rrs gene. Antimicrobial Agents and Chemotherapy53(12), 5064
Enarson, D. A. and Harries, A. D. (2013). Historical background and global epidemiology of Mycobacterium tuberculosis resistance. In Guidelines for Clinical and Operational Management of Drug-Resistant Tuberculosis. France: International Union Against Tuberculosis and Lung Disease.
Sowajassatakul, A., Prammananan, T., Chaiprasert, A. and Phunpruch, S. (2014). Molecular characterization of amikacin, kanamycin and capreomycin resistance in M/XDR-TB strains isolated in Thailand. BMC Microbiology14(1), 165.10.1186/1471-2180-14-165.
Johnson, R., Streicher, E. M., Gail, E. L., Warren, R. M., Van Helden, P. D. and Victor, T. C. (2009). Drug resistance in Mycobacterium tuberculosis. Current Issues in Molecular Biology8, 97
Hanafi, A. R. and Prasenohadi. (2010).Mekanisme dan diagnosis multidrug resistant tuberculosis (MDR TB). The Indonesian Associantion Againt Tuberculosis (PPTI).
World Health Organization. (2015).Global Tuberculosis Report 2015. World Health Organization. 10.1037/e530172011-002.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.