Effects Analysis Leaf Extract Breadfruit (Artocarpus altilis (Park.) Fosberg) Against Insulin Resistance in Rat (Rattus norvegicus) Obese Study on Cell Morphology of Langerhans Island
Keywords:
A. Extracts altilis, obesity, islands of Langerhans, insulin resistance.Abstract
The state of obesity is closely related to insulin resistance that leads to type 2 diabetes incidence of exposure to high concentrations of glucose cause increased apoptosis of pancreatic Langerhans Island. A. altilis plants have traditionally been used by the people of Indonesia for treating diabetes mellitus. The objective of this study was to determine the concentration of leaf extract of A. altilis which can improve insulin resistance in rats (Rattus norvegicus) who are obese by looking at the morphology of Langerhans islet cells. The method used is eksperimntal laboratory to study design Randomized Controlled Trial (RCT). Rats were divided into five groups, with each group consisting of 5 rats, then given a high-fat meal (open source) for fattening or obese.
Weight measurement to assess that the rats had been obese and the fasting blood sugar (GDP) by using the tool GlukoDR tests to ensure that the mice had increased blood glucose and insulin resistance. Group I as a negative control group, the group II as a positive control group using metformin HCl, group III as 5% of the test group, Group IV as the test group, 10% and V as a test group of 15%. HE staining was done to see the number of islets of Langerhans cells of each group. Data analysis was performed using One Way Anova to see the differences between the groups with 95% confidence level. All data were analyzed with SPSS version 21.0 (SPSS, Inc. Chicago, IL). Results revealed that no difference in the number of Langerhans islet cells in the negative control with extracts of A. altilis 5%, 10% and 15%. (P = 0.000) in which 10% extract has the same effect with metformin and extract 15% had a greater effect of metformin (p = 0.000). In conclusion, the extract A. altilis can increase the number of Langerhans islet cells in obese mice. So it can be taken into consideration A. altilis use in the prevention of insulin resistance.
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