Molecular Analysis of rrs Gene Mutation on Detecting The Resistance of Kanamycin from the Clinical Isolates of Tuberculosis Patient

Authors

  • Muhammad Anwar Postgraduate, Medical Faculty, Hasanuddin University, Makassar, 90245, Indonesia.
  • Muhammad Nasrum Massi Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar, Indonesia.
  • Andi Asadul Islam Department of Neurological Surgery, Medical Faculty, Hasanuddin University, Makassar, Indonesia.
  • Irawaty Djaharuddin Department of Pulmonology and Respiratory, Medical Faculty, Hasanuddin University, Makassar, Indonesia.
  • HandayaniHalik HandayaniHalik Medical Research Center, Medical Faculty, Hasanuddin University, Makassar, Indonesia.
  • Gaby Maulida Nurdin Medical Research Center, Medical Faculty, Hasanuddin University, Makassar, Indonesia.

Keywords:

Molecular, rrs Gene Mutation, Resistance of Kanamycin, Tuberculosis Patient.

Abstract

The resistance of kanamycin (KAN) as a second-line drug treatment for tuberculosis is associated with mutations in the 16S rRNA gene (rrsgene).rrsgene mutations occur at different locus causing changes in codons and amino acids.This study aimed to analyze mutations in the rrs gene and its relationship with the resistance of KANin isolate clinical sample ofMycobacterium tuberculosis(MTB). The isolate clinical sample of MTB was obtained from the TB HUM-RC laboratory. The analysis of rrs gene mutation was conducted using PCR sequencing.The results described that from 93 samples isolates, 51 (54.8%) samples shown multidrug resistant (MDR) while 42 samples shown (45.2%) non-MDR. Drug sensitivity test (DST) from the second line drugs samples isolates7 of 93 samples (7.5%) that shown resistance with KAN.

The result of PCR sequencing shown6 (6.4%) of 93 isolates samples had mutations rrsgenein the locus of T1521C (1.1%), G1484C (1.1%), A1401G (2.1%), G940T (1.1%), and A514C (1.1%). Based on that gene locus, an unreported new mutation in the locus of rrs gene, called G940T,was discovered.The results of statistical analysis based on the number of mutations in each of anti-tuberculosis drugs indicate the p<0.05,described a relatioship between the mutation of rrs gene with the resistance of KAN. In conclusion, significance and impact of study, there is an association between the mutation in the rrs gene and the resistance to the second-line drugs of KAN in clinical isolates of MTB.Thus, samples of clinical isolates of MTB could be used to detect any resistance of KAN in advance.

References

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Published

2017-01-20

How to Cite

Anwar, M., Massi, M. N., Islam, A. A., Djaharuddin, I., HandayaniHalik, H., & Nurdin, G. M. (2017). Molecular Analysis of rrs Gene Mutation on Detecting The Resistance of Kanamycin from the Clinical Isolates of Tuberculosis Patient. International Journal of Sciences: Basic and Applied Research (IJSBAR), 31(1), 417–424. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/6831

Issue

Vol. 31 No. 1 (2017)

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Articles

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