Detection Salmonella Typhi RNA from Suspect Typhoid using Reverse Transcription-Polymerase Chain Reaction

Abstract

In endemic areas, approximately 90% of enteric fever is typhoid fever. Diagnosis of typhoid fever is difficult to be enforced only on the basis of clinical symptoms alone, because the clinical features of this disease is very varied and generally not typical. Thus the role of the laboratory in assisting the diagnosis is very important. The aim of this research is to detection of Salmonella typhi from suspected typhoid RNA using Reverse Transcription-Polymerase Chain Reaction. The design of this study is cross-sectional analytical survey. The total sample of 101 patients. Data analysis was done using the Chi square test with a confidence level of 95%. All the data were Analyzed by SPSS software, version 21.0 (SPSS, Inc., Chicago, IL). The result showed no association temperature with the results of PCR (p = 0.004), there is a correlation incident dirty tongue with the results of the PCR (p = 0.000), there is a relationship incidence of headache with the results of the PCR (p = 0.027), there is a relationship incidence of dizziness the results of the PCR (p = 0.035), and no association epistaxis events with the results of the PCR (p = 0.024).

As for the other characteristics: age (p = 0.226), gender (p = 0.819), duration of fever (p = 0.268), fever afternoon (p = 0.579), fever continuously (p = 0.389), cough (p = 1.000), insomnia (p = 0.631), decreased consciousness (p = 1.000), psychosis (p = 1.000), weak (p = 0.314), nausea (p = 1.000), abdominal pain (p = 0.547), anorexia (p = 0.266), constipation (p = 1.000), and diarrhea (p = 0.512) was not associated with the results of PCR. In conclusion, there is a relationship of temperature, dirty tongue, headache, dizziness, and epistaxis with positive PCR test results.