Detection of Mycobacterium bovis by Multiplex Polymerase Chain Reaction in Dairy Cattle in Bogor, West Java, Indonesia
Keywords:
Mycobacterium bovis, Mycobacterium tuberculosis, multiplex PCR.Abstract
Zoonotic tuberculosis (TB) could be a major threat for public health, but there is no prior information of zoonotic TB incidence in cattle in Indonesia. Definitive detection of Mycobacterium bovis and Mycobacterium tuberculosis up to species level is time consuming and difficult, due to close genetic relationship and variable biochemical patterns. Current method, such as multiplex polymerase chain reaction (PCR), could be the best alternative strategy to meet this purpose.
The aims of this study were to determine the prevalence of bovine tuberculosis (BTB), and detect Mycobacterium bovis as a causal of Tuberculosis (TB) in dairy cattle by multiplex PCR method. This research was conducted by sampling the cattle in Kawasan Usaha Peternakan/KUNAK (dairy farm region center), in Bogor District and Kebon Pedes (Bogor City). The single intradermal tuberculin test was conducted in all sampling cattle using Bovituber
References
L.A. Keating, P.R. Wheeler, H. Mansoor, J.K. Inwald, J. Dale, R.G. Hewinson, S.V. Gordon.
I. Olsen, R.G. Barletta, C. Thoen.
R. Lake, S. Gilbert, T.L. Wong, P. Cressey. Risk pro?le: Mycobacterium bovis in milk. Christchurch (NZ): Institute of Environmental, Science and Research, 2009, pp. 1-11.
R. de la Rua-Domenech.
J.M. Grange, M.D. Yates.
O. Cosivi, J.M. Grange, C.J. Daborn, M..C Raviglione, T. Fujikura, D. Cousins, R.A. Robinson, H.F.A.K. Huchzermeyer, I. de Kantor, F.X. Meslin.
W.Y. Ayele, S.D. Neill, J. Zinsstag, M.G. Weiss, I. Pavlik.
L. P
J. Oloya, J. Opuda-Asibo, R. Kazwala, A.B. Demelash, E. Skjerve, A. Lund, T.B. Johansen, B. Dj
S. Cadmus, S. Palmer, M. Okker, J. Dale, K. Gover, N. Smith, K. Jahans, R..G Hewinson, S.V. Gordon.
R. Jou, W.L. Huang, C.Y. Chiang.
F. Byarugaba, E.M.C. Etter, S. Godreuil, P. Grimaud.
A.L. Michel, B. M
C. Thoen, P. Lobue, I. de Kantor.
I. Dohoo, W. Martin, H. Stryhn. Veterinary Epidemiologic Research. Prince Edward Island (CA): AVC, 2003, pp. 140-150, 335-372.
A. Thakur, M. Sharma, V.C. Katoch, P. Dhar, R.C. Katoch.
[OIE] World Organization for Animal Health.
C.S. Bakshi, D.H. Shah, R. Verma, R.K. Singh, M. Malik.
M.K. Omer, E. Skjerve, Z. Woldehiwet, G. Holstad.
A. Mishra, A. Singhai, D.S. Chauhan, V..M Katoch, K. Srivastava, S.S. Thakral, S.S. Bharadwaj, V. Sreenivas, H.K. Prasad.
A.F. Nassar, S. Miyashiro, C.G. Oliveira, W.A. Pacheco, R.A. Ogata.
S. Berg, R. Firdessa, M. Habtamu, E. Gadisa, A. Mengistu, L. Yamuah, G. Ameni, M. Vordermeier, B.D. Robertson, N.H. Smith, H. Engers, D. Young, R.G. Hewinson, A. Aseffa, S.V. Gordon.
Y. Chen, Y. Chao, Q. Deng, T. Liu, J. Xiang, J. Chen, J. Zhou, Z. Zhan, Y. Kuang, H. Cai, H. Chen, A. Guo.
D. Hughes.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.
