Animal Modeling Try Strain Balb/c Mice With Gardnerella Vaginalis

Abstract

Gardnerella vaginalis is the causative agent of sexually transmitted infections (STIs) of the bacterial class and is the main species of bacterial vaginosis (BV), classified as gram-negative bacteria, and is anaerobic facultative. The incidence of bacterial vaginosis increased rapidly in Indonesia with a prevalence of approximately 20-30% of women of childbearing age suffering from sexually transmitted infections diagnosed BV and an increase of 50-60%. BV is the leading cause of vaginal infection in women of childbearing age and is one of the most common infectious diseases in the gynecological obstetrics. Balb-C is a trying animal with a human-like cervical vagina. Objective: To identify the dose of Gardnerella vaginalis bacteria that has the potential to live on the vagina of experimental animals. Methods: Balb/c female strain, adult age 8-12 weeks, weight 20-25 g. Using the patient's urine specimen suspect Bacterial vaginosis was inoculated on intravaginal Balb-c each of 10 ?l doses of 3 x 10⁶, 3 x 10⁵, and 3 x 10⁴. Prior to inoculation (H0), a vaginal swab was taken and continued at H1