Cytotoxic Effects of Anethum graveolensis Extracts Against Some Cell Lines Invitro Study
Keywords:
Anethum graveolensis, Cytotoxicity, cancerous cell line.Abstract
Anethum graveolens L. commonly known as dill belonging to the family Umbelliferae, is one of the most useful essential oil bearing spices as well as medicinal herb. The present study aimed to study the effect of Iraqi traditional medical plants extracts using tissue culture teqnique stud their cytotoxicity in human hepatocarcinoma HepG2 and mouse cell L20B cell lines. Anethum graveolensis leaves were extracted using Aqueous and 99% and alcoholic extraction. eight crude concentration were made by serial dilution, with concentrations of 3.9, 7.81, 15.62, 31.25, 62.5, 125,250 and 500 mg /ml, respectively. These were added in triplicate to the microtiter plate containing 1x105 cells/well and 200 µl of the medium. The eight concentrations were used in triplicate to investigate their cytotoxic and anti-proliferative effects. The alcoholic extracts of Anethum graveolensis showed the highest potent cytotoxicity in the HepG2 and L20B cell lines.while aqueas extracts showed the lowest cytotoxicity. All concentrations of crude extracts showed different cytotoxic activity in vitro.
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