Microelectrode Array for the Dielectrophoretic Orientation of C2C12 Myoblast Cells

Authors

  • Douglas Hanson Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
  • Charles Baker Southwest Research Institute, 6220 Culebra Road, San Antonio, TX 78238, USA
  • Arturo Ayon Department of Physics and Astronomy, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
  • Waldemar Gorski Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA

Keywords:

dielectrophoresis, tissue alignment.

Abstract

An array of interdigitated microelectrodes was modeled, optimized, and tested for the contactless alignment of living biological cells by negative dielectrophoresis (nDEP).  The modeling focused on optimizing the x component, , of potential gradient to maximize the aligning force at minimal electric field to protect fragile cells.  The optimized array was tested with micron-sized, hard polymeric beads and soft C2C12 mouse myoblast cells.  The array completely aligned the initially random arrangement of beads after 15 s of applying 450 kHz sinewave electrical signal to the electrodes.  Under the same conditions, the array was unable to move C2C12 cells due to their strong adhesion to the surface.  However, the array was effective at directing the orientation of confluent C2C12 cells during their differentiation into myotubes for the formation of muscle fibers.  The aligned myotubes were alive and capable of growth.  The proposed approach has a potential for growing continuously oriented viable tissues under the array of microelectrodes.

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Published

2017-09-30

How to Cite

Hanson, D., Baker, C., Ayon, A., & Gorski, W. (2017). Microelectrode Array for the Dielectrophoretic Orientation of C2C12 Myoblast Cells. International Journal of Sciences: Basic and Applied Research (IJSBAR), 36(2), 171–181. Retrieved from https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/8132

Issue

Vol. 36 No. 2 (2017)

Section

Articles

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