Molecular Identification of Endophytic Fungi from Rice (Oryza sativa L.) and its Anatagonistic Effect Against Staphylococcus aureus and Escherichia coli

Abstract

Endophytes are organisms living inside the tissue of the host organism. Most of them usually do not cause detrimental effect to its host species. The study aimed to isolate and identify endophytic fungi from the leaves and roots of rice plant, and evaluates their antagonistic effect against Staphylococcus aureus BIOTECH 158 and Escherichia coli BIOTECH 1634 bacteria. Identification of the collected endophytic fungi was done through PCR based approach using the internal transcribed spacer region of the ITS gene sequence. Three fungi were collected, isolated and were identified as Anthostomella leucospermi (EU552100.1), Ceriporia lacerata (KP689233.1) and Fusarium equiseti (KT277307.1). The isolates Anthostomella leucospermi and Fusarium equiseti have growth inhibition against Staphylococcus aureus and Escherichia coli bacteria. On the otherhand, the growth of Ceriporia lacerata was not inhibited by E. coli but inhibited by S. aureus. The potentials of endophytes that have antibacterial property in this study are good source of important bioactive compounds and are important for discovery of drug components also important for industry and in agricultural and biotechnological research.