Detection of Salmonella Typhi in Urine of 6-12 Years Old Children in the Coastal Region of Southeast Sulawesi Using the Polymerase Chain Reaction Method

  • Salfitriana Rebecha Lakawa Medical Study Program, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Wa Ode Salma Department of Nutrition, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Yenti Purnamasari Microbiology Laboratory of Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Asriati Asriati Department of Epidemiology, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Irawaty Irawaty Department of Clinical Pathology, Faculty of Medicine,, Halu Oleo University, Kendari Indonesia
  • Adius kusnan Department of Nursing, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Sri Susanty Department of Nursing, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
  • Rahmawati Rahmawati Department of Nursing, Faculty of Medicine, Halu Oleo University, Kendari Indonesia
Keywords: Salmonella typhi, children, Urine, PCR.

Abstract

Typhoid fever caused by Salmonella typhi bacteria still become a global health problem throughout the world, especially in developing countries. Previous study reports showed that Salmonella typhi is one of the causes of urinary tract infections in children which suspected of lack of access to clean water, contaminated food and poor environmental sanitation. This study aimed to detect Salmonella typhi bacteria in the urine of children at aged 6-12 years, residing in the Coastal Region of Southeast Sulawesi Province, Indonesia. Measuring instrument to detect Salmonella typhi bacteria in 50 mid-stream urine while in children using the Polymerase Chain Reaction (PCR) method with amplification product length was 302 bp. As much as 47 samples of Nutrient Broth (NB) were positive (94%) and only 3 (6%) samples gave a negative result. The results of the study showed that from 94% of the urine samples of children, Salmonella typhi was detected in their urine as much as 24% with a distribution of 18% female and 6% male.

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Published
2019-04-18
Section
Articles